CPEBs: a family of related RNA-binding proteins involved in distinct steps of spermatogenesis. Export

@inproceedings{citeulike:3313120, abstract = {Translational control via 3'UTRs is critical in the germline of many species. In Xenopus , an RNA-binding protein, CPEB (Cytoplasmic Polyadenylation Element Binding Protein), binds to 3' UTRs of specific maternal mRNAs and is thought to regulate their translation. The C. elegans genome contains 4 putative CPEB homologs. The similarity is greatest in the region that contains 2 RNA Recognition Motifs (RRMs) and a novel C/H-containing Zn-binding domain, both of which are needed for Xenopus CPEB to bind RNA. Using RNAi, we find that one CPEB homolog, cpb-1 , is required for spermatogenesis in both hermaphrodites and males; cpb-1(RNAi) animals have arrested primary spermatocytes and lack secondary spermatocytes or mature sperm. Wild-type males produce viable cross-progeny when mated to the cpb-1(RNAi) hermaphrodites; thus cpb-1 is not required for oogenesis. cpb-1 mRNA is germline-specific. CPB-1 protein is detected in the meiotic region of the L4 germline, but not in adults. Using CPB-1 as bait in the two-hybrid system, we recovered FBF, a repressor of fem-3 required for the sperm/oocyte switch; the interaction's significance is being investigated. A second homolog encodes fog-1 , a gene required to specify sperm fate. The homolog maps to the fog-1 locus, and RNAi directed against the gene results in a feminized (Fog) germ line. We sequenced this gene from a fog-1(q253) mutant and found an amino acid substitution in a residue highly conserved among CPEBs, confirming that it is the fog-1 gene (also see Jin et al abstract). Using FOG-1 as bait in a two-hybrid screen, we've found one specific partner to date; analysis of this partner will be presented. In every species examined, CPEBs are implicated in oogenesis. However, in C. elegans , animals subjected to RNAi against all CPEB homologs simultaneously are Fog. Moreover, oocytes of these animals support embryogenesis and produce viable cross-progeny. Thus CPEBs are dispensable for oogenesis in C. elegans . This is the first report of CPEBs having a role in spermatogenesis, and contrasts with the roles of Xenopus CPEB and Drosophila orb . Worm CPEBs are required for two steps in spermatogenesis: fog-1 specifies the sperm fate, and cpb-1 implements that decision. We suggest that CPB-1 and FOG-1 bind to and regulate specific mRNAs to execute these functions.}, author = {Luitjens, C. M. and Kimble, J. E. and Wickens, M. P.}, citeulike-article-id = {3313120}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00023306}, journal = {International C. elegans Meeting}, keywords = {c01f64, c40h11, c\_elegans, caenorhabditis\_elegans, celegans, cpb-1, elegans, fem-3, fog-1, meeting\_abstract, nematode, wormbase, y54e10a4}, posted-at = {2008-09-22 04:10:37}, priority = {3}, title = {CPEBs: a family of related RNA-binding proteins involved in distinct steps of spermatogenesis.}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00023306}, year = {1999} }

TY - CONF ID - citeulike:3313120 L3 - citeulike-article-id:3313120 N2 - Translational control via 3'UTRs is critical in the germline of many species. In Xenopus , an RNA-binding protein, CPEB (Cytoplasmic Polyadenylation Element Binding Protein), binds to 3' UTRs of specific maternal mRNAs and is thought to regulate their translation. The C. elegans genome contains 4 putative CPEB homologs. The similarity is greatest in the region that contains 2 RNA Recognition Motifs (RRMs) and a novel C/H-containing Zn-binding domain, both of which are needed for Xenopus CPEB to bind RNA. Using RNAi, we find that one CPEB homolog, cpb-1 , is required for spermatogenesis in both hermaphrodites and males; cpb-1(RNAi) animals have arrested primary spermatocytes and lack secondary spermatocytes or mature sperm. Wild-type males produce viable cross-progeny when mated to the cpb-1(RNAi) hermaphrodites; thus cpb-1 is not required for oogenesis. cpb-1 mRNA is germline-specific. CPB-1 protein is detected in the meiotic region of the L4 germline, but not in adults. Using CPB-1 as bait in the two-hybrid system, we recovered FBF, a repressor of fem-3 required for the sperm/oocyte switch; the interaction's significance is being investigated. A second homolog encodes fog-1 , a gene required to specify sperm fate. The homolog maps to the fog-1 locus, and RNAi directed against the gene results in a feminized (Fog) germ line. We sequenced this gene from a fog-1(q253) mutant and found an amino acid substitution in a residue highly conserved among CPEBs, confirming that it is the fog-1 gene (also see Jin et al abstract). Using FOG-1 as bait in a two-hybrid screen, we've found one specific partner to date; analysis of this partner will be presented. In every species examined, CPEBs are implicated in oogenesis. However, in C. elegans , animals subjected to RNAi against all CPEB homologs simultaneously are Fog. Moreover, oocytes of these animals support embryogenesis and produce viable cross-progeny. Thus CPEBs are dispensable for oogenesis in C. elegans . This is the first report of CPEBs having a role in spermatogenesis, and contrasts with the roles of Xenopus CPEB and Drosophila orb . Worm CPEBs are required for two steps in spermatogenesis: fog-1 specifies the sperm fate, and cpb-1 implements that decision. We suggest that CPB-1 and FOG-1 bind to and regulate specific mRNAs to execute these functions. JF - International C. elegans Meeting TI - CPEBs: a family of related RNA-binding proteins involved in distinct steps of spermatogenesis. KW - c01f64 KW - c40h11 KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - cpb-1 KW - elegans KW - fem-3 KW - fog-1 KW - meeting_abstract KW - nematode KW - wormbase KW - y54e10a4 AU - Luitjens, CM AU - Kimble, JE AU - Wickens, MP PY - 1999/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00023306 ER -