Misexpression of acetylcholinesterases in the C. elegans pha-2 mutant accompanies ultrastructural defects in pharyngeal muscle cells. Export

@article{citeulike:3325763, abstract = {pha-2 is the Caenorhabditis elegans homolog of the vertebrate homeobox gene Hex. Embryonic expression of pha-2 is mostly pharyngeal and the only described mutant allele of pha-2 results in a severe pharyngeal defect in which certain muscle cells (pm5 cells) and neurons are grossly deformed. Here, we performed a detailed characterization of the pha-2 phenotype using cell-type-specific reporters, physical manipulation of the nuclei in pharyngeal muscle cells using "optical tweezers", electron microscopy, staining of the actin cytoskeleton as well as phenotypic rescue and ectopic expression experiments. The main findings of the present study are (i) the pha-2 (ad472) mutation specifically impairs the pharyngeal expression of pha-2; (ii) in the pha-2 mutant, the cytoskeleton of the pm5 cells is measurably weaker than in normal cells and is severely disrupted by large tubular structures and organelles; (iii) the pm5 cells of the pha-2 mutant fail to express the acetylcholinesterase genes ace-1 and ace-2; (iv) ectopic expression of pha-2 can induce ectopic expression of ace-1 and ace-2; and (v) the anc-1 mutant with mislocalized pm5 cell nuclei occasionally shows an isthmus phenotype similar to that of pha-2 worms.}, author = {Morck, C. and Axang, C. and Goksor, M. and Pilon, M.}, citeulike-article-id = {3325763}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/16806153}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=16806153}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00027717}, journal = {Dev Biol}, keywords = {ace-1, ace-2, ace-3, ace-4, anc-1, c\_elegans, caenorhabditis\_elegans, celegans, elegans, m63, nematode, other, pha-2, w09b121, wormbase, y44e3a2, y48b6a7, y48b6a8, zk9736}, pages = {446--460}, posted-at = {2008-09-23 22:54:01}, priority = {3}, title = {Misexpression of acetylcholinesterases in the C. elegans pha-2 mutant accompanies ultrastructural defects in pharyngeal muscle cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/16806153}, volume = {297}, year = {2006} }

TY - JOUR ID - citeulike:3325763 L3 - citeulike-article-id:3325763 N2 - pha-2 is the Caenorhabditis elegans homolog of the vertebrate homeobox gene Hex. Embryonic expression of pha-2 is mostly pharyngeal and the only described mutant allele of pha-2 results in a severe pharyngeal defect in which certain muscle cells (pm5 cells) and neurons are grossly deformed. Here, we performed a detailed characterization of the pha-2 phenotype using cell-type-specific reporters, physical manipulation of the nuclei in pharyngeal muscle cells using "optical tweezers", electron microscopy, staining of the actin cytoskeleton as well as phenotypic rescue and ectopic expression experiments. The main findings of the present study are (i) the pha-2 (ad472) mutation specifically impairs the pharyngeal expression of pha-2; (ii) in the pha-2 mutant, the cytoskeleton of the pm5 cells is measurably weaker than in normal cells and is severely disrupted by large tubular structures and organelles; (iii) the pm5 cells of the pha-2 mutant fail to express the acetylcholinesterase genes ace-1 and ace-2; (iv) ectopic expression of pha-2 can induce ectopic expression of ace-1 and ace-2; and (v) the anc-1 mutant with mislocalized pm5 cell nuclei occasionally shows an isthmus phenotype similar to that of pha-2 worms. JF - Dev Biol EP - 460 TI - Misexpression of acetylcholinesterases in the C. elegans pha-2 mutant accompanies ultrastructural defects in pharyngeal muscle cells. VL - 297 SP - 446 KW - ace-1 KW - ace-2 KW - ace-3 KW - ace-4 KW - anc-1 KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - elegans KW - m63 KW - nematode KW - other KW - pha-2 KW - w09b121 KW - wormbase KW - y44e3a2 KW - y48b6a7 KW - y48b6a8 KW - zk9736 AU - Morck, C AU - Axang, C AU - Goksor, M AU - Pilon, M PY - 2006/// UR - http://view.ncbi.nlm.nih.gov/pubmed/16806153 ER -