Serotonin and FLP-1 Neuropeptide Signaling Mediate the Enhanced Slowing Response Export

@article{citeulike:3326359, abstract = {Food-deprived C. elegans slow their locomotion upon encountering bacteria more than do well-fed animals. This behavior, called the enhanced slowing response, is partly dependent on serotonin (5-HT). Animals mutant for the 5-HT reuptake transporter gene mod-5 slow even more than wild-type animals (the hyperenhanced slowing response), presumably because 5-HT signaling is potentiated. mod-5 mutants are also hypersensitive to immobilization by exogenous 5-HT. To identify additional genes involved in 5-HT signaling and possibly in the enhanced slowing response, we screened for suppressors of the 5-HT hypersensitivity of mod-5 animals. We also used a candidate-gene approach, testing the 5-HT resistance of strains containing deletions in metabotropic 5-HT receptor-like genes. We screened 105,000 genomes for suppressors of the 5-HT hypersensitivity of mod-5 mutants. We isolated 26 mutants, which define at least eight genes. Nineteen of these mutants also suppress the hyperenhanced slowing response of mod-5 animals. We identified alleles of two genes known to suppress the 5-HT hypersensitivity and the hyperenhanced slowing response of mod-5 mutants: goa-1, which encodes an alpha subunit of a G-protein, and mod-1, which encodes a 5-HT-gated Cl- channel. We also identified alleles of eat-16, which encodes a Regulator of G protein Signaling (RGS) protein; abts-1, a bicarbonate transporter gene; and flp-1, a neuropeptide gene. Using a candidate-gene approach, we found that two deletion alleles of the gene ser-4 confer 5-HT resistance and defects in the enhanced slowing response. ser-4 encodes a metabotropic 5-HT receptor (Olde and McCombie, J. Mol. Neurosci., 1997). Like goa-1 and mod-1 mutations, eat-16, flp-1 and ser-4 mutations suppress the hyperenhanced slowing response of mod-5 animals. Thus, these genes likely act downstream of or in parallel to one or more 5-HT synapse(s) that mediate(s) the enhanced slowing response. We found that the MOD-1 and SER-4 5-HT receptors act in parallel in the enhanced slowing response. We are currently testing genetic interactions with flp-1 to determine how neuropeptide signaling relates to these two 5-HT signaling pathways. In addition, we are determining where these genes are expressed to identify the neural circuit(s) involved in the enhanced slowing response.}, author = {Higginbotham, Megan and Horvitz, Bob}, citeulike-article-id = {3326359}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00028134}, journal = {Neuronal Development, Synaptic Function, and Behavior Meeting}, keywords = {abts-1, c16c22, c26c62, c\_elegans, caenorhabditis\_elegans, celegans, dmo-1, eat-16, elegans, f23b25, f52b51, flp-1, goa-1, k06c46, meeting\_abstract, mod-1, mod-5, nematode, ser-4, wormbase, y22d7ar13, y54e10br7}, posted-at = {2008-09-24 00:52:06}, priority = {3}, title = {Serotonin and FLP-1 Neuropeptide Signaling Mediate the Enhanced Slowing Response}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00028134}, year = {2006} }

TY - JOUR ID - citeulike:3326359 L3 - citeulike-article-id:3326359 N2 - Food-deprived C. elegans slow their locomotion upon encountering bacteria more than do well-fed animals. This behavior, called the enhanced slowing response, is partly dependent on serotonin (5-HT). Animals mutant for the 5-HT reuptake transporter gene mod-5 slow even more than wild-type animals (the hyperenhanced slowing response), presumably because 5-HT signaling is potentiated. mod-5 mutants are also hypersensitive to immobilization by exogenous 5-HT. To identify additional genes involved in 5-HT signaling and possibly in the enhanced slowing response, we screened for suppressors of the 5-HT hypersensitivity of mod-5 animals. We also used a candidate-gene approach, testing the 5-HT resistance of strains containing deletions in metabotropic 5-HT receptor-like genes. We screened 105,000 genomes for suppressors of the 5-HT hypersensitivity of mod-5 mutants. We isolated 26 mutants, which define at least eight genes. Nineteen of these mutants also suppress the hyperenhanced slowing response of mod-5 animals. We identified alleles of two genes known to suppress the 5-HT hypersensitivity and the hyperenhanced slowing response of mod-5 mutants: goa-1, which encodes an alpha subunit of a G-protein, and mod-1, which encodes a 5-HT-gated Cl- channel. We also identified alleles of eat-16, which encodes a Regulator of G protein Signaling (RGS) protein; abts-1, a bicarbonate transporter gene; and flp-1, a neuropeptide gene. Using a candidate-gene approach, we found that two deletion alleles of the gene ser-4 confer 5-HT resistance and defects in the enhanced slowing response. ser-4 encodes a metabotropic 5-HT receptor (Olde and McCombie, J. Mol. Neurosci., 1997). Like goa-1 and mod-1 mutations, eat-16, flp-1 and ser-4 mutations suppress the hyperenhanced slowing response of mod-5 animals. Thus, these genes likely act downstream of or in parallel to one or more 5-HT synapse(s) that mediate(s) the enhanced slowing response. We found that the MOD-1 and SER-4 5-HT receptors act in parallel in the enhanced slowing response. We are currently testing genetic interactions with flp-1 to determine how neuropeptide signaling relates to these two 5-HT signaling pathways. In addition, we are determining where these genes are expressed to identify the neural circuit(s) involved in the enhanced slowing response. JF - Neuronal Development, Synaptic Function, and Behavior Meeting TI - Serotonin and FLP-1 Neuropeptide Signaling Mediate the Enhanced Slowing Response KW - abts-1 KW - c16c22 KW - c26c62 KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - dmo-1 KW - eat-16 KW - elegans KW - f23b25 KW - f52b51 KW - flp-1 KW - goa-1 KW - k06c46 KW - meeting_abstract KW - mod-1 KW - mod-5 KW - nematode KW - ser-4 KW - wormbase KW - y22d7ar13 KW - y54e10br7 AU - Higginbotham, Megan AU - Horvitz, Bob PY - 2006/// UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00028134 ER -