Sequence and genetic map of Meloidogyne hapla: A compact nematode genome for plant parasitism. Export

@article{citeulike:3334071, abstract = {We have established Meloidogyne hapla as a tractable model plant-parasitic nematode amenable to forward and reverse genetics, and we present a complete genome sequence. At 54 Mbp, M. hapla represents not only the smallest nematode genome yet completed, but also the smallest metazoan, and defines a platform to elucidate mechanisms of parasitism by what is the largest uncontrolled group of plant pathogens worldwide. The M. hapla genome encodes significantly fewer genes than does the free-living nematode Caenorhabditis elegans (most notably through a reduction of odorant receptors and other gene families), yet it has acquired horizontally from other kingdoms numerous genes suspected to be involved in adaptations to parasitism. In some cases, amplification and tandem duplication have occurred with genes suspected of being acquired horizontally and involved in parasitism of plants. Although M. hapla and C. elegans diverged >500 million years ago, many developmental and biochemical pathways, including those for dauer formation and RNAi, are conserved. Although overall genome organization is not conserved, there are areas of microsynteny that may suggest a primary biological function in nematodes for those genes in these areas. This sequence and map represent a wealth of biological information on both the nature of nematode parasitism of plants and its evolution.}, address = {Center for the Biology of Nematode Parasitism, Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695;}, author = {Opperman, Charles H. and Bird, David M. and Williamson, Valerie M. and Rokhsar, Dan S. and Burke, Mark and Cohn, Jonathan and Cromer, John and Diener, Steve and Gajan, Jim and Graham, Steve and Houfek, T. D. and Liu, Qingli and Mitros, Therese and Schaff, Jennifer and Schaffer, Reenah and Scholl, Elizabeth and Sosinski, Bryon R. and Thomas, Varghese P. and Windham, Eric}, citeulike-article-id = {3334071}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0805946105}, citeulike-linkout-1 = {http://www.pnas.org/content/105/39/14802.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/39/14802.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/18809916}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=18809916}, day = {30}, doi = {10.1073/pnas.0805946105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {genome, nematode, parasite, sequence}, month = {September}, number = {39}, pages = {14802--14807}, posted-at = {2009-09-14 16:56:23}, priority = {2}, title = {Sequence and genetic map of Meloidogyne hapla: A compact nematode genome for plant parasitism.}, url = {http://dx.doi.org/10.1073/pnas.0805946105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:3334071 L3 - citeulike-article-id:3334071 N2 - We have established Meloidogyne hapla as a tractable model plant-parasitic nematode amenable to forward and reverse genetics, and we present a complete genome sequence. At 54 Mbp, M. hapla represents not only the smallest nematode genome yet completed, but also the smallest metazoan, and defines a platform to elucidate mechanisms of parasitism by what is the largest uncontrolled group of plant pathogens worldwide. The M. hapla genome encodes significantly fewer genes than does the free-living nematode Caenorhabditis elegans (most notably through a reduction of odorant receptors and other gene families), yet it has acquired horizontally from other kingdoms numerous genes suspected to be involved in adaptations to parasitism. In some cases, amplification and tandem duplication have occurred with genes suspected of being acquired horizontally and involved in parasitism of plants. Although M. hapla and C. elegans diverged >500 million years ago, many developmental and biochemical pathways, including those for dauer formation and RNAi, are conserved. Although overall genome organization is not conserved, there are areas of microsynteny that may suggest a primary biological function in nematodes for those genes in these areas. This sequence and map represent a wealth of biological information on both the nature of nematode parasitism of plants and its evolution. IS - 39 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 1091-6490 AD - Center for the Biology of Nematode Parasitism, Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695; EP - 14807 TI - Sequence and genetic map of Meloidogyne hapla: A compact nematode genome for plant parasitism. VL - 105 SP - 14802 KW - genome KW - nematode KW - parasite KW - sequence AU - Opperman, Charles AU - Bird, David AU - Williamson, Valerie AU - Rokhsar, Dan AU - Burke, Mark AU - Cohn, Jonathan AU - Cromer, John AU - Diener, Steve AU - Gajan, Jim AU - Graham, Steve AU - Houfek, TD AU - Liu, Qingli AU - Mitros, Therese AU - Schaff, Jennifer AU - Schaffer, Reenah AU - Scholl, Elizabeth AU - Sosinski, Bryon AU - Thomas, Varghese AU - Windham, Eric PY - 2008/09/30/ UR - http://dx.doi.org/10.1073/pnas.0805946105 ER -