Caenorhabditis elegans Intersectin: A Synaptic Protein Regulating Neurotransmission. Export

@article{citeulike:3334611, abstract = {Monitoring Editor: Sandra Schmid Intersectin is a multifunctional protein that interacts with components of the endocytic and exocytic pathways and is also involved in the control of actin dynamics. Drosophila intersectin is required for viability, synaptic development and synaptic vesicle recycling. Here, we report the characterization of intersectin function in C. elegans. Nematode intersectin (ITSN-1) is expressed in the nervous system, and it is enriched in presynaptic regions. The C. elegans intersectin gene (itsn-1) is nonessential for viability. In addition, itsn-1-null worms do not display any evident phenotype, under physiological conditions. However, they display aldicarb-hypersensitivity, compatible with a negative regulatory role of ITSN-1 on neurotransmission. ITSN-1 physically interacts with dynamin and EHS-1, two proteins involved in synaptic vesicle recycling. We have previously shown that EHS-1 is a positive modulator of synaptic vesicle recycling in the nematode, likely through modulation of dynamin or dynamin-controlled pathways. Here, we show that ITSN-1 and EHS-1 have opposite effects on aldicarb sensitivity, and on dynamin-dependent phenotypes. Thus, the sum of our results identifies dynamin, or a dynamin-controlled pathway, as a potential target for the negative regulatory role of ITSN-1.}, author = {Rose, S. and Malabarba, M. G. and Krag, C. and Schultz, A. and Tsushima, H. and Di Fiore, P. P. and Salcini, A. E.}, citeulike-article-id = {3334611}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/17942601}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=17942601}, citeulike-linkout-2 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031112}, journal = {Mol Biol Cell}, keywords = {ace-1, ace-2, c02c61, c52e122, c\_elegans, caenorhabditis\_elegans, celegans, dyn-1, ehs-1, elegans, itsn-1, nematode, other, snb-1, t04d13, t10h94, unc-104, unc-57, w09b121, wormbase, y116a8c36, y44e3a2, zk12483}, posted-at = {2008-09-25 04:18:17}, priority = {3}, title = {Caenorhabditis elegans Intersectin: A Synaptic Protein Regulating Neurotransmission.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17942601}, year = {2007} }

TY - JOUR ID - citeulike:3334611 L3 - citeulike-article-id:3334611 N2 - Monitoring Editor: Sandra Schmid Intersectin is a multifunctional protein that interacts with components of the endocytic and exocytic pathways and is also involved in the control of actin dynamics. Drosophila intersectin is required for viability, synaptic development and synaptic vesicle recycling. Here, we report the characterization of intersectin function in C. elegans. Nematode intersectin (ITSN-1) is expressed in the nervous system, and it is enriched in presynaptic regions. The C. elegans intersectin gene (itsn-1) is nonessential for viability. In addition, itsn-1-null worms do not display any evident phenotype, under physiological conditions. However, they display aldicarb-hypersensitivity, compatible with a negative regulatory role of ITSN-1 on neurotransmission. ITSN-1 physically interacts with dynamin and EHS-1, two proteins involved in synaptic vesicle recycling. We have previously shown that EHS-1 is a positive modulator of synaptic vesicle recycling in the nematode, likely through modulation of dynamin or dynamin-controlled pathways. Here, we show that ITSN-1 and EHS-1 have opposite effects on aldicarb sensitivity, and on dynamin-dependent phenotypes. Thus, the sum of our results identifies dynamin, or a dynamin-controlled pathway, as a potential target for the negative regulatory role of ITSN-1. JF - Mol Biol Cell TI - Caenorhabditis elegans Intersectin: A Synaptic Protein Regulating Neurotransmission. KW - ace-1 KW - ace-2 KW - c02c61 KW - c52e122 KW - c_elegans KW - caenorhabditis_elegans KW - celegans KW - dyn-1 KW - ehs-1 KW - elegans KW - itsn-1 KW - nematode KW - other KW - snb-1 KW - t04d13 KW - t10h94 KW - unc-104 KW - unc-57 KW - w09b121 KW - wormbase KW - y116a8c36 KW - y44e3a2 KW - zk12483 AU - Rose, S AU - Malabarba, MG AU - Krag, C AU - Schultz, A AU - Tsushima, H AU - Di Fiore, PP AU - Salcini, AE PY - 2007/// UR - http://view.ncbi.nlm.nih.gov/pubmed/17942601 ER -