Distinctive roles of four CDC25 family members during germline development in C. elegans Export

@inproceedings{citeulike:3337305, abstract = {"For the cell cycle regulator, CDC25, there are four C. elegans homologs, CDC-25.1 to CDC-25.4. CDC-25.1 is known as an important key player during G1/S transition. It is maternally loaded into embryos and consequently essential for embryogenesis. In addition, post-embryonically, it functions exclusively in germline development as deletion allele of cdc-25.1, nr2036, was previously reported to exhibit sterility due to defective germ cell proliferation while somatic post-embryonic development was not affected. These results suggest that CDC-25.1 is essential for germline development and other family members of CDC25 may be responsible for somatic cell divisions. To investigate these possibilities we examined functions of CDC-25.1, 2, 3, and 4 by characterizing its mutant and RNAi phenocopies. cdc-25.2, cdc-25.3 mutant alleles showed sterility, in which germ cell proliferation occurred unlike cdc-25.1 but germ cell differentiation was defective. RNAi of cdc-25.4 showed similar phenocopy. These results indicate that all four CDC-25 family members of C. elegans are required for germline development with distinctive roles of each member but not for the post-embryonic soma development. Analysis for expression levels of four cdc-25 mRNA supported this hypothesis as their levels are dependent on the number of germ cells, oocytes and sperm, respectively. Further study showed that CDC-25 family members are interacting with WEE-1.3 during the germline development. We obtained some genetic data suggesting that CDC-25 family members and WEE-1.3 may coordinately regulate germline development at several different steps. This study was supported by Forest Science and Technology project (S110707L0501101) and the second BK21 (MOE)"}, author = {Kim, Ji-Young and Kawasaki, Ichiro and Shim, Yhong-Hee}, citeulike-article-id = {3337305}, citeulike-linkout-0 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031796}, keywords = {c\_elegans, caenorhabditis\_elegans, cdc-251, cdc-252, cdc-253, cdc-254, celegans, elegans, f16b48, k06a57, meeting\_abstract, nematode, r05h52, wormbase, zk63711}, posted-at = {2008-09-25 17:25:50}, priority = {3}, title = {Distinctive roles of four CDC25 family members during germline development in C. elegans}, url = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031796} }

TY - CONF ID - citeulike:3337305 L3 - citeulike-article-id:3337305 N2 - "For the cell cycle regulator, CDC25, there are four C. elegans homologs, CDC-25.1 to CDC-25.4. CDC-25.1 is known as an important key player during G1/S transition. It is maternally loaded into embryos and consequently essential for embryogenesis. In addition, post-embryonically, it functions exclusively in germline development as deletion allele of cdc-25.1, nr2036, was previously reported to exhibit sterility due to defective germ cell proliferation while somatic post-embryonic development was not affected. These results suggest that CDC-25.1 is essential for germline development and other family members of CDC25 may be responsible for somatic cell divisions. To investigate these possibilities we examined functions of CDC-25.1, 2, 3, and 4 by characterizing its mutant and RNAi phenocopies. cdc-25.2, cdc-25.3 mutant alleles showed sterility, in which germ cell proliferation occurred unlike cdc-25.1 but germ cell differentiation was defective. RNAi of cdc-25.4 showed similar phenocopy. These results indicate that all four CDC-25 family members of C. elegans are required for germline development with distinctive roles of each member but not for the post-embryonic soma development. Analysis for expression levels of four cdc-25 mRNA supported this hypothesis as their levels are dependent on the number of germ cells, oocytes and sperm, respectively. Further study showed that CDC-25 family members are interacting with WEE-1.3 during the germline development. We obtained some genetic data suggesting that CDC-25 family members and WEE-1.3 may coordinately regulate germline development at several different steps. This study was supported by Forest Science and Technology project (S110707L0501101) and the second BK21 (MOE)" TI - Distinctive roles of four CDC25 family members during germline development in C. elegans KW - c_elegans KW - caenorhabditis_elegans KW - cdc-251 KW - cdc-252 KW - cdc-253 KW - cdc-254 KW - celegans KW - elegans KW - f16b48 KW - k06a57 KW - meeting_abstract KW - nematode KW - r05h52 KW - wormbase KW - zk63711 AU - Kim, Ji-Young AU - Kawasaki, Ichiro AU - Shim, Yhong-Hee UR - http://www.wormbase.org/db/misc/paper?name=WBPaper00031796 ER -