Patient-Specific Embryonic Stem Cells Derived from Human SCNT Blastocysts.

@article{citeulike:230856, abstract = {Patient-specific, immune-matched human embryonic stem cells (hESC) are anticipated to be of great biomedical importance for studies of disease and development, and to advance clinical deliberations for stem cell transplantation. Eleven hESC lines were established by nuclear transfer (SCNT; NT) of skin cells from patients with disease or injury into donated oocytes. These lines (NT-hESCs), grown on human feeders from the same NT-donor or genetically-unrelated individuals, were established at high rates, regardless of NT-donor sex or age. NT-hESCs were pluripotent, chromosomally normal, and match NT-patient's DNA. Major Histocompatibility Complex (MHC) identity of each NT-hESC with the patient's showed immunological compatibility, important for eventual transplantation. With the generation of these NT-hESCs, evaluations of genetic and epigenetic stability can be made. Additional work remains regarding the development of reliable directed differentiation and the elimination of remaining animal components. Prior to clinical use of these cells, preclinical evidence is required to prove that transplantation of differentiated-NT-hESCs can be safe, effective and tolerated.}, address = {College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea; School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.}, author = {Hwang, Woo Suk S. and Roh, Sung Il I. and Lee, Byeong Chun C. and Kang, Sung Keun K. and Kwon, Dae Kee K. and Kim, Sue and Kim, Sun Jong J. and Park, Sun Woo W. and Kwon, Hee Sun S. and Lee, Chang Kyu K. and Lee, Jung Bok B. and Kim, Jin Mee M. and Ahn, Curie and Paek, Sun Ha H. and Chang, Sang Sik S. and Koo, Jung Jin J. and Yoon, Hyun Soo S. and Hwang, Jung Hye H. and Hwang, Youn Young Y. and Park, Ye Soo S. and Oh, Sun Kyung K. and Kim, Hee Sun S. and Park, Jong Hyuk H. and Moon, Shin Yong Y. and Schatten, Gerald }, citeulike-article-id = {230856}, comment = {2004년 논문이 가짜니까 2005년 논문도 자연히 가짜가 되었네 }, doi = {http://dx.doi.org/10.1126/science.1112286}, issn = {1095-9203}, journal = {Science}, keywords = {scnt}, month = {May}, posted-at = {2008-05-05 03:15:34}, priority = {2}, title = {Patient-Specific Embryonic Stem Cells Derived from Human SCNT Blastocysts.}, url = {http://dx.doi.org/10.1126/science.1112286}, year = {2005} }

TY - JOUR ID - citeulike:230856 L3 - citeulike-article-id:230856 TI - Patient-Specific Embryonic Stem Cells Derived from Human SCNT Blastocysts. JF - Science AD - College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea; School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea. SN - 1095-9203 N2 - Patient-specific, immune-matched human embryonic stem cells (hESC) are anticipated to be of great biomedical importance for studies of disease and development, and to advance clinical deliberations for stem cell transplantation. Eleven hESC lines were established by nuclear transfer (SCNT; NT) of skin cells from patients with disease or injury into donated oocytes. These lines (NT-hESCs), grown on human feeders from the same NT-donor or genetically-unrelated individuals, were established at high rates, regardless of NT-donor sex or age. NT-hESCs were pluripotent, chromosomally normal, and match NT-patient's DNA. Major Histocompatibility Complex (MHC) identity of each NT-hESC with the patient's showed immunological compatibility, important for eventual transplantation. With the generation of these NT-hESCs, evaluations of genetic and epigenetic stability can be made. Additional work remains regarding the development of reliable directed differentiation and the elimination of remaining animal components. Prior to clinical use of these cells, preclinical evidence is required to prove that transplantation of differentiated-NT-hESCs can be safe, effective and tolerated. KW - scnt N1 - 2004년 논문이 가짜니까 2005년 논문도 자연히 가짜가 되었네 AU - Hwang, Woo Suk AU - Roh, Sung Il AU - Lee, Byeong Chun AU - Kang, Sung Keun AU - Kwon, Dae Kee AU - Kim, Sue AU - Kim, Sun Jong AU - Park, Sun Woo AU - Kwon, Hee Sun AU - Lee, Chang Kyu AU - Lee, Jung Bok AU - Kim, Jin Mee AU - Ahn, Curie AU - Paek, Sun Ha AU - Chang, Sang Sik AU - Koo, Jung Jin AU - Yoon, Hyun Soo AU - Hwang, Jung Hye AU - Hwang, Youn Young AU - Park, Ye Soo AU - Oh, Sun Kyung AU - Kim, Hee Sun AU - Park, Jong Hyuk AU - Moon, Shin Yong AU - Schatten, Gerald PY - 2005/05/19/ UR - http://dx.doi.org/10.1126/science.1112286 ER -