In vivo bone formation from human embryonic stem cell-derived osteogenic cells in poly(d,l-lactic-co-glycolic acid)/hydroxyapatite composite scaffolds. Export

@article{citeulike:2638671, abstract = {We have previously reported the efficient osteogenic differentiation of human embryonic stem cells (hESCs) by co-culture with primary human bone-derived cells (hPBDs) without the use of exogenous factors. In the present study, we explored whether osteogenic cells derived from hESCs (OC-hESCs) using the previously reported method would be capable of regenerating bone tissue in vivo. A three-dimensional porous poly(d,l-lactic-co-glycolic acid)/hydroxyapatite composite scaffold was used as a cell delivery vehicle. In vivo implantation of OC-hESC-seeded scaffolds showed significant bone formation in the subcutaneous sites of immunodeficient mice at 4 and 8 weeks after implantation (n=5 for each time point). Meanwhile, implantation of the control no cell-seeded scaffolds or human dermal fibroblast-seeded scaffolds did not show any new bone formation. In addition, the presence of BMP-2 (1 microg/scaffold) enhanced new bone tissue formation in terms of mineralization and the expression of bone-specific genetic markers. According to FISH analysis, implanted OC-hESCs remained in the regeneration sites, which suggested that the implanted cells participated in the formation of new bone. In conclusion, OC-hESCs successfully regenerated bone tissue upon in vivo implantation, and this regeneration can be further enhanced by the administration of BMP-2. These results suggest the clinical feasibility of OC-hESCs as a good alternative source of cells for bone regeneration.}, address = {Graduate School of Life Science, CHA Stem Cell Institute, Pochon CHA University, 606-16 Yoeksam 1-dong, Gangnam-gu, Seoul 135-081, Republic of Korea.}, author = {Kim, S. and Kim, S. S. and Lee, S. H. and Eun Ahn, S. and Gwak, S. J. and Song, J. H. and Kim, B. S. and Chung, H. M.}, citeulike-article-id = {2638671}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.biomaterials.2007.11.005}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18023477}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18023477}, doi = {10.1016/j.biomaterials.2007.11.005}, issn = {0142-9612}, journal = {Biomaterials}, keywords = {chasc}, month = {March}, number = {8}, pages = {1043--1053}, posted-at = {2008-04-07 21:09:08}, priority = {2}, title = {In vivo bone formation from human embryonic stem cell-derived osteogenic cells in poly(d,l-lactic-co-glycolic acid)/hydroxyapatite composite scaffolds.}, url = {http://dx.doi.org/10.1016/j.biomaterials.2007.11.005}, volume = {29}, year = {2008} }

TY - JOUR ID - citeulike:2638671 L3 - citeulike-article-id:2638671 N2 - We have previously reported the efficient osteogenic differentiation of human embryonic stem cells (hESCs) by co-culture with primary human bone-derived cells (hPBDs) without the use of exogenous factors. In the present study, we explored whether osteogenic cells derived from hESCs (OC-hESCs) using the previously reported method would be capable of regenerating bone tissue in vivo. A three-dimensional porous poly(d,l-lactic-co-glycolic acid)/hydroxyapatite composite scaffold was used as a cell delivery vehicle. In vivo implantation of OC-hESC-seeded scaffolds showed significant bone formation in the subcutaneous sites of immunodeficient mice at 4 and 8 weeks after implantation (n=5 for each time point). Meanwhile, implantation of the control no cell-seeded scaffolds or human dermal fibroblast-seeded scaffolds did not show any new bone formation. In addition, the presence of BMP-2 (1 microg/scaffold) enhanced new bone tissue formation in terms of mineralization and the expression of bone-specific genetic markers. According to FISH analysis, implanted OC-hESCs remained in the regeneration sites, which suggested that the implanted cells participated in the formation of new bone. In conclusion, OC-hESCs successfully regenerated bone tissue upon in vivo implantation, and this regeneration can be further enhanced by the administration of BMP-2. These results suggest the clinical feasibility of OC-hESCs as a good alternative source of cells for bone regeneration. IS - 8 JF - Biomaterials SN - 0142-9612 AD - Graduate School of Life Science, CHA Stem Cell Institute, Pochon CHA University, 606-16 Yoeksam 1-dong, Gangnam-gu, Seoul 135-081, Republic of Korea. EP - 1053 TI - In vivo bone formation from human embryonic stem cell-derived osteogenic cells in poly(d,l-lactic-co-glycolic acid)/hydroxyapatite composite scaffolds. VL - 29 SP - 1043 KW - chasc AU - Kim, S AU - Kim, SS AU - Lee, SH AU - Eun Ahn, S AU - Gwak, SJ AU - Song, JH AU - Kim, BS AU - Chung, HM PY - 2008/03// UR - http://dx.doi.org/10.1016/j.biomaterials.2007.11.005 ER -