Auditory hair cell explant co-cultures promote the differentiation of stem cells into bipolar neurons. Export

@article{citeulike:2639489, abstract = {Auditory neurons, the target neurons of the cochlear implant, degenerate following a sensorineural hearing loss. The goal of this research is to direct the differentiation of embryonic stem cells (SCs) into bipolar auditory neurons that can be used to replace degenerating neurons in the deafened mammalian cochlea. Successful replacement of auditory neurons is likely to result in improved clinical outcomes for cochlear implant recipients. We examined two post-natal auditory co-culture models with and without neurotrophic support, for their potential to direct the differentiation of mouse embryonic SCs into characteristic, bipolar, auditory neurons. The differentiation of SCs into neuron-like cells was facilitated by co-culture with auditory neurons or hair cell explants, isolated from post-natal day five rats. The most successful combination was the co-culture of hair cell explants with whole embryoid bodies, which resulted in significantly greater numbers of neurofilament-positive, neuron-like cells. While further characterization of these differentiated cells will be essential before transplantation studies commence, these data illustrate the effectiveness of post-natal hair cell explant co-culture, at providing valuable molecular cues for directed differentiation of SCs towards an auditory neuron lineage.}, address = {Department of Otolaryngology, University of Melbourne, East Melbourne, Australia. bcoleman@bionicear.org}, author = {Coleman, B. and Fallon, J. B. and Pettingill, L. N. and de Silva, M. G. and Shepherd, R. K.}, citeulike-article-id = {2639489}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.yexcr.2006.10.010}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17112512}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17112512}, citeulike-linkout-3 = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17112512}, citeulike-linkout-4 = {http://ebeen.com/life/43}, citeulike-linkout-5 = {http://dx.doi.org.ezp1.harvard.edu/10.1016/j.yexcr.2006.10.010}, day = {15}, doi = {10.1016/j.yexcr.2006.10.010}, issn = {0014-4827}, journal = {Experimental cell research}, keywords = {co-culture, vitro}, month = {January}, number = {2}, pages = {232--243}, posted-at = {2008-04-07 23:35:53}, priority = {2}, title = {Auditory hair cell explant co-cultures promote the differentiation of stem cells into bipolar neurons.}, url = {http://dx.doi.org/10.1016/j.yexcr.2006.10.010}, volume = {313}, year = {2007} }

TY - JOUR ID - citeulike:2639489 L3 - citeulike-article-id:2639489 N2 - Auditory neurons, the target neurons of the cochlear implant, degenerate following a sensorineural hearing loss. The goal of this research is to direct the differentiation of embryonic stem cells (SCs) into bipolar auditory neurons that can be used to replace degenerating neurons in the deafened mammalian cochlea. Successful replacement of auditory neurons is likely to result in improved clinical outcomes for cochlear implant recipients. We examined two post-natal auditory co-culture models with and without neurotrophic support, for their potential to direct the differentiation of mouse embryonic SCs into characteristic, bipolar, auditory neurons. The differentiation of SCs into neuron-like cells was facilitated by co-culture with auditory neurons or hair cell explants, isolated from post-natal day five rats. The most successful combination was the co-culture of hair cell explants with whole embryoid bodies, which resulted in significantly greater numbers of neurofilament-positive, neuron-like cells. While further characterization of these differentiated cells will be essential before transplantation studies commence, these data illustrate the effectiveness of post-natal hair cell explant co-culture, at providing valuable molecular cues for directed differentiation of SCs towards an auditory neuron lineage. IS - 2 JF - Experimental cell research SN - 0014-4827 AD - Department of Otolaryngology, University of Melbourne, East Melbourne, Australia. bcoleman@bionicear.org EP - 243 TI - Auditory hair cell explant co-cultures promote the differentiation of stem cells into bipolar neurons. VL - 313 SP - 232 KW - co-culture KW - vitro AU - Coleman, B AU - Fallon, JB AU - Pettingill, LN AU - de Silva, MG AU - Shepherd, RK PY - 2007/01/15/ UR - http://dx.doi.org/10.1016/j.yexcr.2006.10.010 ER -