Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst

@article{citeulike:550091, abstract = {Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.}, author = {Hwang, Woo S. and Ryu, Young J. and Park, Jong H. and Park, Eul S. and Lee, Eu G. and Koo, Ja M. and Jeon, Hyun Y. and Lee, Byeong C. and Kang, Sung K. and Kim, Sun J. and Ahn, Curie and Hwang, Jung H. and Park, Ky Y. and Cibelli, Jose B. and Moon, Shin Y.}, citeulike-article-id = {550091}, doi = {10.1126/science.1094515}, journal = {Science}, keywords = {scnt}, month = {March}, number = {5664}, pages = {1669--1674}, posted-at = {2008-05-05 02:31:38}, priority = {2}, title = {Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst}, url = {http://dx.doi.org/10.1126/science.1094515}, volume = {303}, year = {2004} }

TY - JOUR ID - citeulike:550091 L3 - citeulike-article-id:550091 N2 - Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells. IS - 5664 JF - Science EP - 1674 TI - Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst VL - 303 SP - 1669 KW - scnt AU - Hwang, Woo AU - Ryu, Young AU - Park, Jong AU - Park, Eul AU - Lee, Eu AU - Koo, Ja AU - Jeon, Hyun AU - Lee, Byeong AU - Kang, Sung AU - Kim, Sun AU - Ahn, Curie AU - Hwang, Jung AU - Park, Ky AU - Cibelli, Jose AU - Moon, Shin PY - 2004/03/12/ UR - http://dx.doi.org/10.1126/science.1094515 ER -