5-Fluorouracil ointment for the treatment of otitis media with effusion. Export

@article{citeulike:6082055, abstract = {OBJECTIVES/HYPOTHESIS: Our aim was to evaluate the combined effect of 5-fluorouracil (5-FU) and myringotomy for the treatment of otitis media with effusion (OME). OME is usually treated with medication, myringotomy, or insertion of a ventilation tube (VT). Except for VT insertion, however, treatment effects are short-lived. VT insertion has numerous sequelae: increased susceptibility to infection, large perforation of the tympanic membrane, cholesteatoma, and eventual hearing deterioration. We estimated the depressant action of 5-FU on normal cell proliferation in vitro. In addition, clinically, we assessed whether 5-FU has the potential to prolong the effect of myringotomy. STUDY DESIGN: An in vitro study and a clinical study were conducted. MATERIALS AND METHODS: In study I, fibroblasts harvested from the peritoneum of three green fluorescent protein transgenic mice were cultured with different doses of 5-FU. After 2 weeks, their proliferation rates were compared. In study II, patients (54 males, 47 females) were selected randomly from a group of patients with intractable OME. Myringotomy with or without a single dose of 5-FU ointment (approximately 0.10-0.30 mg) was performed in group I (n = 64) and group II (n = 37), respectively. The natural closure rates of the tympanic membrane were assessed in both groups. RESULTS: In vitro, 5-FU inhibited the growth of fibroblasts in a dose-dependent manner. The average time to tympanic membrane closure was 20.5 days in group I and 8.1 days in group II. No adverse events were observed in either group. CONCLUSIONS: Topical application of 5-FU ointment is useful in prolonging the effect of myringotomy. 5-FU ointment therapy is easy, safe, and cost-effective and may be of wide application.}, author = {Kanemaru, Shin-ichi and Nakamura, Tatsuo and Yamashita, Masaru and Magrfov, Akhmar and Omori, Koichi and Ito, Juichi}, citeulike-article-id = {6082055}, citeulike-linkout-0 = {http://dx.doi.org/10.1097/01.mlg.0000248231.62834.e3}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17277614}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17277614}, doi = {10.1097/01.mlg.0000248231.62834.e3}, issn = {0023-852X}, journal = {The Laryngoscope}, keywords = {lm}, month = {February}, number = {2}, pages = {215--219}, posted-at = {2009-11-07 09:14:28}, priority = {2}, title = {5-Fluorouracil ointment for the treatment of otitis media with effusion.}, url = {http://dx.doi.org/10.1097/01.mlg.0000248231.62834.e3}, volume = {117}, year = {2007} }

TY - JOUR ID - citeulike:6082055 L3 - citeulike-article-id:6082055 N2 - OBJECTIVES/HYPOTHESIS: Our aim was to evaluate the combined effect of 5-fluorouracil (5-FU) and myringotomy for the treatment of otitis media with effusion (OME). OME is usually treated with medication, myringotomy, or insertion of a ventilation tube (VT). Except for VT insertion, however, treatment effects are short-lived. VT insertion has numerous sequelae: increased susceptibility to infection, large perforation of the tympanic membrane, cholesteatoma, and eventual hearing deterioration. We estimated the depressant action of 5-FU on normal cell proliferation in vitro. In addition, clinically, we assessed whether 5-FU has the potential to prolong the effect of myringotomy. STUDY DESIGN: An in vitro study and a clinical study were conducted. MATERIALS AND METHODS: In study I, fibroblasts harvested from the peritoneum of three green fluorescent protein transgenic mice were cultured with different doses of 5-FU. After 2 weeks, their proliferation rates were compared. In study II, patients (54 males, 47 females) were selected randomly from a group of patients with intractable OME. Myringotomy with or without a single dose of 5-FU ointment (approximately 0.10-0.30 mg) was performed in group I (n = 64) and group II (n = 37), respectively. The natural closure rates of the tympanic membrane were assessed in both groups. RESULTS: In vitro, 5-FU inhibited the growth of fibroblasts in a dose-dependent manner. The average time to tympanic membrane closure was 20.5 days in group I and 8.1 days in group II. No adverse events were observed in either group. CONCLUSIONS: Topical application of 5-FU ointment is useful in prolonging the effect of myringotomy. 5-FU ointment therapy is easy, safe, and cost-effective and may be of wide application. IS - 2 JF - The Laryngoscope SN - 0023-852X EP - 219 TI - 5-Fluorouracil ointment for the treatment of otitis media with effusion. VL - 117 SP - 215 KW - lm AU - Kanemaru, Shin-ichi AU - Nakamura, Tatsuo AU - Yamashita, Masaru AU - Magrfov, Akhmar AU - Omori, Koichi AU - Ito, Juichi PY - 2007/02// UR - http://dx.doi.org/10.1097/01.mlg.0000248231.62834.e3 ER -