An estimate of divergence time of Parazoa and Eumetazoa and that of Cephalochordata and Vertebrata by aldolase and triose phosphate isomerase clocks. Export

@article{citeulike:684557, abstract = {Previously we suggested that four proteins including aldolase and triose phosphate isomerase (TPI) evolved with approximately constant rates over long periods covering the whole animal phyla. The constant rates of aldolase and TPI evolution were reexamined based on three different models for estimating evolutionary distances. It was shown that the evolutionary rates remain essentially unchanged in comparisons not only between different classes of vertebrates but also between vertebrates and arthropods and even between animals and plants, irrespective of the models used. Thus these enzymes might be useful molecular clocks for inferring divergence times of animal phyla. To know the divergence time of Parazoa and Eumetazoa and that of Cephalochordata and Vertebrata, the aldolase cDNAs from Ephydatia fluviatilis, a freshwater sponge, and the TPI cDNAs from Ephydatia fluviatilis and Branchiostoma belcheri, an amphioxus, have been cloned and sequenced. Comparisons of the deduced amino acid sequences of aldolase and TPI from the freshwater sponge with known sequences revealed that the Parazoa-Eumetazoa split occurred about 940 million years ago (Ma) as determined by the average of two proteins and three models. Similarly, the aldolase and TPI clocks suggest that vertebrates and amphioxus last shared a common ancestor around 700 Ma and they possibly diverged shortly after the divergence of deuterostomes and protostomes.}, address = {Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606, Japan.}, author = {Nikoh, N. and Iwabe, N. and Kuma, K. and Ohno, M. and Sugiyama, T. and Watanabe, Y. and Yasui, K. and Shi-cui, Z. and Hori, K. and Shimura, Y. and Miyata, T.}, citeulike-article-id = {684557}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9211740}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9211740}, comment = {Genes with clock-like behavior}, issn = {0022-2844}, journal = {J Mol Evol}, keywords = {divergence-times}, month = {July}, number = {1}, pages = {97--106}, posted-at = {2006-06-05 17:08:21}, priority = {4}, title = {An estimate of divergence time of Parazoa and Eumetazoa and that of Cephalochordata and Vertebrata by aldolase and triose phosphate isomerase clocks.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9211740}, volume = {45}, year = {1997} }

TY - JOUR ID - citeulike:684557 L3 - citeulike-article-id:684557 N2 - Previously we suggested that four proteins including aldolase and triose phosphate isomerase (TPI) evolved with approximately constant rates over long periods covering the whole animal phyla. The constant rates of aldolase and TPI evolution were reexamined based on three different models for estimating evolutionary distances. It was shown that the evolutionary rates remain essentially unchanged in comparisons not only between different classes of vertebrates but also between vertebrates and arthropods and even between animals and plants, irrespective of the models used. Thus these enzymes might be useful molecular clocks for inferring divergence times of animal phyla. To know the divergence time of Parazoa and Eumetazoa and that of Cephalochordata and Vertebrata, the aldolase cDNAs from Ephydatia fluviatilis, a freshwater sponge, and the TPI cDNAs from Ephydatia fluviatilis and Branchiostoma belcheri, an amphioxus, have been cloned and sequenced. Comparisons of the deduced amino acid sequences of aldolase and TPI from the freshwater sponge with known sequences revealed that the Parazoa-Eumetazoa split occurred about 940 million years ago (Ma) as determined by the average of two proteins and three models. Similarly, the aldolase and TPI clocks suggest that vertebrates and amphioxus last shared a common ancestor around 700 Ma and they possibly diverged shortly after the divergence of deuterostomes and protostomes. IS - 1 JF - J Mol Evol SN - 0022-2844 AD - Department of Biophysics, Faculty of Science, Kyoto University, Kyoto 606, Japan. EP - 106 TI - An estimate of divergence time of Parazoa and Eumetazoa and that of Cephalochordata and Vertebrata by aldolase and triose phosphate isomerase clocks. VL - 45 SP - 97 KW - divergence-times N1 - Genes with clock-like behavior AU - Nikoh, N AU - Iwabe, N AU - Kuma, K AU - Ohno, M AU - Sugiyama, T AU - Watanabe, Y AU - Yasui, K AU - Shi-cui, Z AU - Hori, K AU - Shimura, Y AU - Miyata, T PY - 1997/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/9211740 ER -