Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose Export

@article{citeulike:4680932, abstract = {The 9E10 antibody epitope (EQKLISEEDL) derives from a protein sequence in the human proto-oncogen p62c-myc and is widely used as a protein fusion tag. This myc-tag is a powerful tool in protein localization, immunochemistry, ELISA or protein purification. Here, we characterize the myc-tag epitope by substitutional analysis and length variation using peptide spot synthesis on cellulose. The key amino acids of this interaction are the core residues LISE. The shortest peptide with a strong binding signal is KLISEEDL. Dissociation constants of selected peptide variants to the antibody 9E10 were determined. scFv constructs with the shortest possible myc-tags were successfully detected by Western blot and ELISA, giving a signal comparable to that of the original myc-tag. 10.1093/protein/14.10.803}, author = {Hilpert, K. and Hansen, G. and Wessner, H. and Kuttner, G. and Welfle, K. and Seifert, M. and Hohne, W.}, citeulike-article-id = {4680932}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/protein/14.10.803}, citeulike-linkout-1 = {http://peds.oxfordjournals.org/cgi/content/abstract/14/10/803}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11739900}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11739900}, doi = {10.1093/protein/14.10.803}, journal = {Protein Eng.}, keywords = {antibodies, myc}, month = {October}, number = {10}, pages = {803--806}, posted-at = {2009-05-30 05:42:53}, priority = {0}, title = {Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose}, url = {http://dx.doi.org/10.1093/protein/14.10.803}, volume = {14}, year = {2001} }

TY - JOUR ID - citeulike:4680932 L3 - citeulike-article-id:4680932 N2 - The 9E10 antibody epitope (EQKLISEEDL) derives from a protein sequence in the human proto-oncogen p62c-myc and is widely used as a protein fusion tag. This myc-tag is a powerful tool in protein localization, immunochemistry, ELISA or protein purification. Here, we characterize the myc-tag epitope by substitutional analysis and length variation using peptide spot synthesis on cellulose. The key amino acids of this interaction are the core residues LISE. The shortest peptide with a strong binding signal is KLISEEDL. Dissociation constants of selected peptide variants to the antibody 9E10 were determined. scFv constructs with the shortest possible myc-tags were successfully detected by Western blot and ELISA, giving a signal comparable to that of the original myc-tag. 10.1093/protein/14.10.803 IS - 10 JF - Protein Eng. EP - 806 TI - Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose VL - 14 SP - 803 KW - antibodies KW - myc AU - Hilpert, K AU - Hansen, G AU - Wessner, H AU - Kuttner, G AU - Welfle, K AU - Seifert, M AU - Hohne, W PY - 2001/10/01/ UR - http://dx.doi.org/10.1093/protein/14.10.803 ER -