Sequence analysis of the MYC oncogene involved in the t(8;14)(q24;q11) chromosome translocation in a human leukemia T-cell line indicates that putative regulatory regions are not altered. Export

@article{citeulike:5875201, abstract = {We have cloned the translocation-associated and homologous normal MYC alleles from SKW-3, a leukemia T-cell line with the t(8;14)(q24;q11) translocation, and determined the sequence of the MYC oncogene first exon and flanking 5' putative regulatory regions. S1 nuclease protection experiments utilizing a MYC first exon probe demonstrated transcriptional deregulation of the MYC gene associated with the T-cell receptor alpha locus on the 8q+ chromosome of SKW-3 cells. Nucleotide sequence analysis of the translocation-associated (8q+) MYC allele identified a single base substitution within the upstream flanking region; the homologous nontranslocated allele contained an additional substitution and a two-base deletion. None of the deletions or substitutions localized to putative 5' regulatory regions. The MYC first exon sequence was germ line in both alleles. These results demonstrate that alterations within the putative 5' MYC regulatory regions are not necessarily involved in MYC deregulation in T-cell leukemias, and they show that juxtaposition of the T-cell receptor alpha locus to a germ-line MYC oncogene results in MYC deregulation.}, author = {Finver, S. N. and Nishikura, K. and Finger, L. R. and Haluska, F. G. and Finan, J. and Nowell, P. C. and Croce, C. M.}, citeulike-article-id = {5875201}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/2834731}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=2834731}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {cancer, leukemia, myc, t-cells, translocation}, month = {May}, number = {9}, pages = {3052--3056}, posted-at = {2009-10-02 09:55:08}, priority = {2}, title = {Sequence analysis of the MYC oncogene involved in the t(8;14)(q24;q11) chromosome translocation in a human leukemia T-cell line indicates that putative regulatory regions are not altered.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/2834731}, volume = {85}, year = {1988} }

TY - JOUR ID - citeulike:5875201 L3 - citeulike-article-id:5875201 N2 - We have cloned the translocation-associated and homologous normal MYC alleles from SKW-3, a leukemia T-cell line with the t(8;14)(q24;q11) translocation, and determined the sequence of the MYC oncogene first exon and flanking 5' putative regulatory regions. S1 nuclease protection experiments utilizing a MYC first exon probe demonstrated transcriptional deregulation of the MYC gene associated with the T-cell receptor alpha locus on the 8q+ chromosome of SKW-3 cells. Nucleotide sequence analysis of the translocation-associated (8q+) MYC allele identified a single base substitution within the upstream flanking region; the homologous nontranslocated allele contained an additional substitution and a two-base deletion. None of the deletions or substitutions localized to putative 5' regulatory regions. The MYC first exon sequence was germ line in both alleles. These results demonstrate that alterations within the putative 5' MYC regulatory regions are not necessarily involved in MYC deregulation in T-cell leukemias, and they show that juxtaposition of the T-cell receptor alpha locus to a germ-line MYC oncogene results in MYC deregulation. IS - 9 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 EP - 3056 TI - Sequence analysis of the MYC oncogene involved in the t(8;14)(q24;q11) chromosome translocation in a human leukemia T-cell line indicates that putative regulatory regions are not altered. VL - 85 SP - 3052 KW - cancer KW - leukemia KW - myc KW - t-cells KW - translocation AU - Finver, SN AU - Nishikura, K AU - Finger, LR AU - Haluska, FG AU - Finan, J AU - Nowell, PC AU - Croce, CM PY - 1988/05// UR - http://view.ncbi.nlm.nih.gov/pubmed/2834731 ER -