Recruitment of activated G protein-coupled receptors to pre-existing clathrin-coated pits in living cells. Export

@article{citeulike:2211132, abstract = {The process of clathrin-mediated endocytosis tightly regulates signaling of the superfamily of seven-transmembrane G protein-coupled receptors (GPCRs). A fundamental question in the cell biology of membrane receptor endocytosis is whether activated receptors can initiate the formation of clathrin-coated pits (CPs) or whether they are simply mobilized to pre-existing CPs. Here, using various approaches, including a dynamic assay to monitor the distribution of CPs and GPCR-beta-arrestin complexes in live HeLa cells, we demonstrate for the first time that activated GPCRs do not initiate the de novo formation of CPs but instead are targeted to pre-existing CPs.}, address = {Department of Cell Biology, Institut Cochin de G\'{e}n\'{e}tique Mol\'{e}culaire, Pavillon Gustave Roussy, 75679 Paris CEDEX 14, France.}, author = {Scott, M. G. and Benmerah, A. and Muntaner, O. and Marullo, S.}, citeulike-article-id = {2211132}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M106586200}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11602587}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11602587}, day = {1}, doi = {10.1074/jbc.M106586200}, issn = {0021-9258}, journal = {J Biol Chem}, keywords = {arrestin, benmerah, scott}, month = {February}, number = {5}, pages = {3552--3559}, posted-at = {2008-01-09 14:44:25}, priority = {2}, title = {Recruitment of activated G protein-coupled receptors to pre-existing clathrin-coated pits in living cells.}, url = {http://dx.doi.org/10.1074/jbc.M106586200}, volume = {277}, year = {2002} }

TY - JOUR ID - citeulike:2211132 L3 - citeulike-article-id:2211132 N2 - The process of clathrin-mediated endocytosis tightly regulates signaling of the superfamily of seven-transmembrane G protein-coupled receptors (GPCRs). A fundamental question in the cell biology of membrane receptor endocytosis is whether activated receptors can initiate the formation of clathrin-coated pits (CPs) or whether they are simply mobilized to pre-existing CPs. Here, using various approaches, including a dynamic assay to monitor the distribution of CPs and GPCR-beta-arrestin complexes in live HeLa cells, we demonstrate for the first time that activated GPCRs do not initiate the de novo formation of CPs but instead are targeted to pre-existing CPs. IS - 5 JF - J Biol Chem SN - 0021-9258 AD - Department of Cell Biology, Institut Cochin de Génétique Moléculaire, Pavillon Gustave Roussy, 75679 Paris CEDEX 14, France. EP - 3559 TI - Recruitment of activated G protein-coupled receptors to pre-existing clathrin-coated pits in living cells. VL - 277 SP - 3552 KW - arrestin KW - benmerah KW - scott AU - Scott, MG AU - Benmerah, A AU - Muntaner, O AU - Marullo, S PY - 2002/02/01/ UR - http://dx.doi.org/10.1074/jbc.M106586200 ER -