The chemokine receptor D6 constitutively traffics to and from the cell surface to internalize and degrade chemokines. Export

@article{citeulike:2870312, abstract = {The D6 heptahelical membrane protein, expressed by lymphatic endothelial cells, is able to bind with high affinity to multiple proinflammatory CC chemokines. However, this binding does not allow D6 to couple to the signaling pathways activated by typical chemokine receptors such as CC-chemokine receptor-5 (CCR5). Here, we show that D6, like CCR5, can rapidly internalize chemokines. However, D6-internalized chemokines are more effectively retained intracellularly because they more readily dissociate from the receptor during vesicle acidification. These chemokines are then degraded while the receptor recycles to the cell surface. Interestingly, D6-mediated chemokine internalization occurs without bringing about a reduction in cell surface D6 levels. This is possible because unlike CCR5, D6 is predominantly localized in recycling endosomes capable of trafficking to and from the cell surface in the absence of ligand. When chemokine is present, it can enter the cells associated with D6 already destined for internalization. By this mechanism, D6 can target chemokines for degradation without the necessity for cell signaling, and without desensitizing the cell to subsequent chemokine exposure.}, address = {The Cancer Research UK Beatson Laboratories, The Beatson Institute for Cancer Research, Glasgow G61 1BD, Scotland, UK.}, author = {Weber, M. and Blair, E. and Simpson, C. V. and O'Hara, M. and Blackburn, P. E. and Rot, A. and Graham, G. J. and Nibbs, R. J.}, citeulike-article-id = {2870312}, citeulike-linkout-0 = {http://dx.doi.org/10.1091/mbc.E03-09-0634}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15004236}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15004236}, doi = {10.1091/mbc.E03-09-0634}, issn = {1059-1524}, journal = {Molecular biology of the cell}, keywords = {clathrin, d6, intro}, month = {May}, number = {5}, pages = {2492--2508}, posted-at = {2008-06-06 20:51:18}, priority = {2}, title = {The chemokine receptor D6 constitutively traffics to and from the cell surface to internalize and degrade chemokines.}, url = {http://dx.doi.org/10.1091/mbc.E03-09-0634}, volume = {15}, year = {2004} }

TY - JOUR ID - citeulike:2870312 L3 - citeulike-article-id:2870312 N2 - The D6 heptahelical membrane protein, expressed by lymphatic endothelial cells, is able to bind with high affinity to multiple proinflammatory CC chemokines. However, this binding does not allow D6 to couple to the signaling pathways activated by typical chemokine receptors such as CC-chemokine receptor-5 (CCR5). Here, we show that D6, like CCR5, can rapidly internalize chemokines. However, D6-internalized chemokines are more effectively retained intracellularly because they more readily dissociate from the receptor during vesicle acidification. These chemokines are then degraded while the receptor recycles to the cell surface. Interestingly, D6-mediated chemokine internalization occurs without bringing about a reduction in cell surface D6 levels. This is possible because unlike CCR5, D6 is predominantly localized in recycling endosomes capable of trafficking to and from the cell surface in the absence of ligand. When chemokine is present, it can enter the cells associated with D6 already destined for internalization. By this mechanism, D6 can target chemokines for degradation without the necessity for cell signaling, and without desensitizing the cell to subsequent chemokine exposure. IS - 5 JF - Molecular biology of the cell SN - 1059-1524 AD - The Cancer Research UK Beatson Laboratories, The Beatson Institute for Cancer Research, Glasgow G61 1BD, Scotland, UK. EP - 2508 TI - The chemokine receptor D6 constitutively traffics to and from the cell surface to internalize and degrade chemokines. VL - 15 SP - 2492 KW - clathrin KW - d6 KW - intro AU - Weber, M AU - Blair, E AU - Simpson, CV AU - O'Hara, M AU - Blackburn, PE AU - Rot, A AU - Graham, GJ AU - Nibbs, RJ PY - 2004/05// UR - http://dx.doi.org/10.1091/mbc.E03-09-0634 ER -