Chemotaxis in a lymphocyte cell line transfected with C-C chemokine receptor 2B: evidence that directed migration is mediated by betagamma dimers released by activation of Galphai-coupled receptors. Export

@article{citeulike:2871390, abstract = {Chemotaxis is mediated by activation of seven-transmembrane domain, G protein-coupled receptors, but the signal transduction pathways leading to chemotaxis are poorly understood. To identify G proteins that signal the directed migration of cells, we stably transfected a lymphocyte cell line (300-19) with G protein-coupled receptors that couple exclusively to Galphaq (the m3 muscarinic receptor), Galphai (the kappa-opioid receptor), and Galphas (the beta-adrenergic receptor), as well as the human thrombin receptor (PAR-1) and the C-C chemokine receptor 2B. Cells expressing receptors that coupled to Galphai, but not to Galphaq or Galphas, migrated in response to a concentration gradient of the appropriate agonist. Overexpression of Galpha transducin, which binds to and inactivates free Gbetagamma dimers, completely blocked chemotaxis although having little or no effect on intracellular calcium mobilization or other measures of cell signaling. The identification of Gbetagamma dimers as a crucial intermediate in the chemotaxis signaling pathway provides further evidence that chemotaxis of mammalian cells has important similarities to polarized responses in yeast. We conclude that chemotaxis is dependent on activation of Galphai and the release of Gbetagamma dimers, and that Galphai-coupled receptors not traditionally associated with chemotaxis can mediate directed migration when they are expressed in hematopoietic cells.}, address = {Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94141-9100, USA.}, author = {Arai, H. and Tsou, C. L. and Charo, I. F.}, citeulike-article-id = {2871390}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9405641}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9405641}, day = {23}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {ccr2, chemotaxis}, month = {December}, number = {26}, pages = {14495--14499}, posted-at = {2008-06-07 15:39:48}, priority = {2}, title = {Chemotaxis in a lymphocyte cell line transfected with C-C chemokine receptor 2B: evidence that directed migration is mediated by betagamma dimers released by activation of Galphai-coupled receptors.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9405641}, volume = {94}, year = {1997} }

TY - JOUR ID - citeulike:2871390 L3 - citeulike-article-id:2871390 N2 - Chemotaxis is mediated by activation of seven-transmembrane domain, G protein-coupled receptors, but the signal transduction pathways leading to chemotaxis are poorly understood. To identify G proteins that signal the directed migration of cells, we stably transfected a lymphocyte cell line (300-19) with G protein-coupled receptors that couple exclusively to Galphaq (the m3 muscarinic receptor), Galphai (the kappa-opioid receptor), and Galphas (the beta-adrenergic receptor), as well as the human thrombin receptor (PAR-1) and the C-C chemokine receptor 2B. Cells expressing receptors that coupled to Galphai, but not to Galphaq or Galphas, migrated in response to a concentration gradient of the appropriate agonist. Overexpression of Galpha transducin, which binds to and inactivates free Gbetagamma dimers, completely blocked chemotaxis although having little or no effect on intracellular calcium mobilization or other measures of cell signaling. The identification of Gbetagamma dimers as a crucial intermediate in the chemotaxis signaling pathway provides further evidence that chemotaxis of mammalian cells has important similarities to polarized responses in yeast. We conclude that chemotaxis is dependent on activation of Galphai and the release of Gbetagamma dimers, and that Galphai-coupled receptors not traditionally associated with chemotaxis can mediate directed migration when they are expressed in hematopoietic cells. IS - 26 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA 94141-9100, USA. EP - 14499 TI - Chemotaxis in a lymphocyte cell line transfected with C-C chemokine receptor 2B: evidence that directed migration is mediated by betagamma dimers released by activation of Galphai-coupled receptors. VL - 94 SP - 14495 KW - ccr2 KW - chemotaxis AU - Arai, H AU - Tsou, CL AU - Charo, IF PY - 1997/12/23/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9405641 ER -