Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells: effects of brefeldin A and G protein activators. Export

@article{citeulike:2936802, abstract = {Brefeldin A (BFA) causes a rapid redistribution of coat proteins (e.g., gamma-adaptin) associated with the clathrin-coated vesicles that bud from the trans-Golgi network (TGN), while the clathrin-coated vesicles that bud from the plasma membrane are unaffected. gamma-Adaptin redistributes with the same kinetics as beta-COP, a coat protein associated with the non-clathrin-coated vesicles that bud from the Golgi complex. Upon removal of BFA, however, gamma-adaptin recovers its perinuclear distribution more rapidly. Redistribution of both proteins can be prevented by pretreating cells with AlF4-. Recruitment of adaptors from the cytosol onto the TGN membrane has been reconstituted in a permeabilized cell system and is increased by addition of GTP gamma S and blocked by addition of BFA. These results suggest a role for G proteins in the control of the clathrin-coated vesicle cycle at the TGN and further extend the similarities between clathrin-coated vesicles and non-clathrin-coated vesicles.}, address = {Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, England.}, author = {Robinson, M. S. and Kreis, T. E.}, citeulike-article-id = {2936802}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/1555237}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=1555237}, day = {3}, issn = {0092-8674}, journal = {Cell}, keywords = {adaptin, bfa, methods}, month = {April}, number = {1}, pages = {129--138}, posted-at = {2008-06-27 12:59:59}, priority = {2}, title = {Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells: effects of brefeldin A and G protein activators.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1555237}, volume = {69}, year = {1992} }

TY - JOUR ID - citeulike:2936802 L3 - citeulike-article-id:2936802 N2 - Brefeldin A (BFA) causes a rapid redistribution of coat proteins (e.g., gamma-adaptin) associated with the clathrin-coated vesicles that bud from the trans-Golgi network (TGN), while the clathrin-coated vesicles that bud from the plasma membrane are unaffected. gamma-Adaptin redistributes with the same kinetics as beta-COP, a coat protein associated with the non-clathrin-coated vesicles that bud from the Golgi complex. Upon removal of BFA, however, gamma-adaptin recovers its perinuclear distribution more rapidly. Redistribution of both proteins can be prevented by pretreating cells with AlF4-. Recruitment of adaptors from the cytosol onto the TGN membrane has been reconstituted in a permeabilized cell system and is increased by addition of GTP gamma S and blocked by addition of BFA. These results suggest a role for G proteins in the control of the clathrin-coated vesicle cycle at the TGN and further extend the similarities between clathrin-coated vesicles and non-clathrin-coated vesicles. IS - 1 JF - Cell SN - 0092-8674 AD - Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, England. EP - 138 TI - Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells: effects of brefeldin A and G protein activators. VL - 69 SP - 129 KW - adaptin KW - bfa KW - methods AU - Robinson, MS AU - Kreis, TE PY - 1992/04/03/ UR - http://view.ncbi.nlm.nih.gov/pubmed/1555237 ER -