Plasmacytoid Dendritic Cell Recruitment by Immobilized CXCR3 Ligands Export

@article{citeulike:3112511, abstract = {Plasmacytoid dendritic cells (pDCs) recognize microbes, viruses in particular, and provide unique means of innate defense against them. The mechanism of pDC tissue recruitment remained enigmatic because the ligands of CXCR3, the cardinal chemokine receptor on pDCs, have failed to induce in vitro chemotaxis of pDCs in the absence of additional chemokines. In this study, we demonstrate that CXCR3 is sufficient to induce pDC migration, however, by a migratory mechanism that amalgamates the features of haptotaxis and chemorepulsion. To mediate "haptorepulsion" of pDCs, CXCR3 requires the encounter of its cognate ligands immobilized, optimally by heparan sulfate, in a form of a negative gradient. This is the first report of the absolute requirement of chemokine immobilization and presentation for its in vitro promigratory activity. The paradigmatic example of pDC haptorepulsion described here may represent a new pathophysiologically relevant migratory mechanism potentially used by other cells in response to other chemokines.}, author = {Kohrgruber, Norbert and Groger, Marion and Meraner, Paul and Kriehuber, Ernst and Petzelbauer, Peter and Brandt, Sabine and Stingl, Georg and Rot, Antal and Maurer, Dieter}, citeulike-article-id = {3112511}, citeulike-linkout-0 = {http://www.jimmunol.org/cgi/content/abstract/173/11/6592}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15557149}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15557149}, day = {1}, journal = {J Immunol}, keywords = {chemotaxis}, month = {December}, number = {11}, pages = {6592--6602}, posted-at = {2008-08-12 19:01:44}, priority = {2}, title = {Plasmacytoid Dendritic Cell Recruitment by Immobilized CXCR3 Ligands}, url = {http://www.jimmunol.org/cgi/content/abstract/173/11/6592}, volume = {173}, year = {2004} }

TY - JOUR ID - citeulike:3112511 L3 - citeulike-article-id:3112511 N2 - Plasmacytoid dendritic cells (pDCs) recognize microbes, viruses in particular, and provide unique means of innate defense against them. The mechanism of pDC tissue recruitment remained enigmatic because the ligands of CXCR3, the cardinal chemokine receptor on pDCs, have failed to induce in vitro chemotaxis of pDCs in the absence of additional chemokines. In this study, we demonstrate that CXCR3 is sufficient to induce pDC migration, however, by a migratory mechanism that amalgamates the features of haptotaxis and chemorepulsion. To mediate "haptorepulsion" of pDCs, CXCR3 requires the encounter of its cognate ligands immobilized, optimally by heparan sulfate, in a form of a negative gradient. This is the first report of the absolute requirement of chemokine immobilization and presentation for its in vitro promigratory activity. The paradigmatic example of pDC haptorepulsion described here may represent a new pathophysiologically relevant migratory mechanism potentially used by other cells in response to other chemokines. IS - 11 JF - J Immunol EP - 6602 TI - Plasmacytoid Dendritic Cell Recruitment by Immobilized CXCR3 Ligands VL - 173 SP - 6592 KW - chemotaxis AU - Kohrgruber, Norbert AU - Groger, Marion AU - Meraner, Paul AU - Kriehuber, Ernst AU - Petzelbauer, Peter AU - Brandt, Sabine AU - Stingl, Georg AU - Rot, Antal AU - Maurer, Dieter PY - 2004/12/01/ UR - http://www.jimmunol.org/cgi/content/abstract/173/11/6592 ER -