Acetohydroxyacid synthase mutations conferring resistance to imidazolinone or sulfonylurea herbicides in sunflower. Export

@article{citeulike:1014533, abstract = {Wild biotypes of cultivated sunflower ( Helianthus annuus L.) are weeds in corn ( Zea mays L.), soybean ( Glycine max L.), and other crops in North America, and are commonly controlled by applying acetohydroxyacid synthase (AHAS)-inhibiting herbicides. Biotypes resistant to two classes of AHAS-inhibiting herbicides-imidazolinones (IMIs) or sulfonylureas (SUs)-have been discovered in wild sunflower populations (ANN-PUR and ANN-KAN) treated with imazethapyr or chlorsulfuron, respectively. The goals of the present study were to isolate AHAS genes from sunflower, identify mutations in AHAS genes conferring herbicide resistance in ANN-PUR and ANN-KAN, and develop tools for marker-assisted selection (MAS) of herbicide resistance genes in sunflower. Three AHAS genes ( AHAS1, AHAS2, and AHAS3) were identified, cloned, and sequenced from herbicide-resistant (mutant) and -susceptible (wild type) genotypes. We identified 48 single-nucleotide polymorphisms (SNPs) in AHAS1, a single six-base pair insertion-deletion in AHAS2, and a single SNP in AHAS3. No DNA polymorphisms were found in AHAS2 among elite inbred lines. AHAS1 from imazethapyr-resistant inbreds harbored a C-to-T mutation in codon 205 ( Arabidopsis thaliana codon nomenclature), conferring resistance to IMI herbicides, whereas AHAS1 from chlorsulfuron-resistant inbreds harbored a C-to-T mutation in codon 197, conferring resistance to SU herbicides. SNP and single-strand conformational polymorphism markers for AHAS1, AHAS2, and AHAS3 were developed and genetically mapped. AHAS1, AHAS2, and AHAS3 mapped to linkage groups 2 ( AHAS3), 6 ( AHAS2), and 9 ( AHAS1). The C/T SNP in codon 205 of AHAS1 cosegregated with a partially dominant gene for resistance to IMI herbicides in two mutant x wild-type populations. The molecular breeding tools described herein create the basis for rapidly identifying new mutations in AHAS and performing MAS for herbicide resistance genes in sunflower.}, address = {Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USA.}, author = {Kolkman, J. M. and Slabaugh, M. B. and Bruniard, J. M. and Berry, S. and Bushman, B. S. and Olungu, C. and Maes, N. and Abratti, G. and Zambelli, A. and Miller, J. F. and Leon, A. and Knapp, S. J.}, citeulike-article-id = {1014533}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00122-004-1716-7}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15309298}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15309298}, doi = {10.1007/s00122-004-1716-7}, issn = {0040-5752}, journal = {Theor Appl Genet}, keywords = {als, mutation, sunflower}, month = {October}, number = {6}, pages = {1147--1159}, posted-at = {2006-12-26 10:52:41}, priority = {2}, title = {Acetohydroxyacid synthase mutations conferring resistance to imidazolinone or sulfonylurea herbicides in sunflower.}, url = {http://dx.doi.org/10.1007/s00122-004-1716-7}, volume = {109}, year = {2004} }

TY - JOUR ID - citeulike:1014533 L3 - citeulike-article-id:1014533 N2 - Wild biotypes of cultivated sunflower ( Helianthus annuus L.) are weeds in corn ( Zea mays L.), soybean ( Glycine max L.), and other crops in North America, and are commonly controlled by applying acetohydroxyacid synthase (AHAS)-inhibiting herbicides. Biotypes resistant to two classes of AHAS-inhibiting herbicides-imidazolinones (IMIs) or sulfonylureas (SUs)-have been discovered in wild sunflower populations (ANN-PUR and ANN-KAN) treated with imazethapyr or chlorsulfuron, respectively. The goals of the present study were to isolate AHAS genes from sunflower, identify mutations in AHAS genes conferring herbicide resistance in ANN-PUR and ANN-KAN, and develop tools for marker-assisted selection (MAS) of herbicide resistance genes in sunflower. Three AHAS genes ( AHAS1, AHAS2, and AHAS3) were identified, cloned, and sequenced from herbicide-resistant (mutant) and -susceptible (wild type) genotypes. We identified 48 single-nucleotide polymorphisms (SNPs) in AHAS1, a single six-base pair insertion-deletion in AHAS2, and a single SNP in AHAS3. No DNA polymorphisms were found in AHAS2 among elite inbred lines. AHAS1 from imazethapyr-resistant inbreds harbored a C-to-T mutation in codon 205 ( Arabidopsis thaliana codon nomenclature), conferring resistance to IMI herbicides, whereas AHAS1 from chlorsulfuron-resistant inbreds harbored a C-to-T mutation in codon 197, conferring resistance to SU herbicides. SNP and single-strand conformational polymorphism markers for AHAS1, AHAS2, and AHAS3 were developed and genetically mapped. AHAS1, AHAS2, and AHAS3 mapped to linkage groups 2 ( AHAS3), 6 ( AHAS2), and 9 ( AHAS1). The C/T SNP in codon 205 of AHAS1 cosegregated with a partially dominant gene for resistance to IMI herbicides in two mutant x wild-type populations. The molecular breeding tools described herein create the basis for rapidly identifying new mutations in AHAS and performing MAS for herbicide resistance genes in sunflower. IS - 6 JF - Theor Appl Genet SN - 0040-5752 AD - Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USA. EP - 1159 TI - Acetohydroxyacid synthase mutations conferring resistance to imidazolinone or sulfonylurea herbicides in sunflower. VL - 109 SP - 1147 KW - als KW - mutation KW - sunflower AU - Kolkman, JM AU - Slabaugh, MB AU - Bruniard, JM AU - Berry, S AU - Bushman, BS AU - Olungu, C AU - Maes, N AU - Abratti, G AU - Zambelli, A AU - Miller, JF AU - Leon, A AU - Knapp, SJ PY - 2004/10// UR - http://dx.doi.org/10.1007/s00122-004-1716-7 ER -