Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Export

@article{citeulike:1024808, abstract = {We generated transgenic mice in which red, green, yellow, or cyan fluorescent proteins (together termed XFPs) were selectively expressed in neurons. All four XFPs labeled neurons in their entirety, including axons, nerve terminals, dendrites, and dendritic spines. Remarkably, each of 25 independently generated transgenic lines expressed XFP in a unique pattern, even though all incorporated identical regulatory elements (from the thyl gene). For example, all retinal ganglion cells or many cortical neurons were XFP positive in some lines, whereas only a few ganglion cells or only layer 5 cortical pyramids were labeled in others. In some lines, intense labeling of small neuronal subsets provided a Golgi-like vital stain. In double transgenic mice expressing two different XFPs, it was possible to differentially label 3 neuronal subsets in a single animal.}, address = {Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA.}, author = {Feng, G. and Mellor, R. H. and Bernstein, M. and Keller-Peck, C. and Nguyen, Q. T. and Wallace, M. and Nerbonne, J. M. and Lichtman, J. W. and Sanes, J. R.}, citeulike-article-id = {1024808}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11086982}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11086982}, issn = {0896-6273}, journal = {Neuron}, keywords = {gfp, mouse, thy1, transgenic}, month = {October}, number = {1}, pages = {41--51}, posted-at = {2007-01-04 13:55:19}, priority = {2}, title = {Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11086982}, volume = {28}, year = {2000} }

TY - JOUR ID - citeulike:1024808 L3 - citeulike-article-id:1024808 N2 - We generated transgenic mice in which red, green, yellow, or cyan fluorescent proteins (together termed XFPs) were selectively expressed in neurons. All four XFPs labeled neurons in their entirety, including axons, nerve terminals, dendrites, and dendritic spines. Remarkably, each of 25 independently generated transgenic lines expressed XFP in a unique pattern, even though all incorporated identical regulatory elements (from the thyl gene). For example, all retinal ganglion cells or many cortical neurons were XFP positive in some lines, whereas only a few ganglion cells or only layer 5 cortical pyramids were labeled in others. In some lines, intense labeling of small neuronal subsets provided a Golgi-like vital stain. In double transgenic mice expressing two different XFPs, it was possible to differentially label 3 neuronal subsets in a single animal. IS - 1 JF - Neuron SN - 0896-6273 AD - Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA. EP - 51 TI - Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. VL - 28 SP - 41 KW - gfp KW - mouse KW - thy1 KW - transgenic AU - Feng, G AU - Mellor, RH AU - Bernstein, M AU - Keller-Peck, C AU - Nguyen, QT AU - Wallace, M AU - Nerbonne, JM AU - Lichtman, JW AU - Sanes, JR PY - 2000/10// UR - http://view.ncbi.nlm.nih.gov/pubmed/11086982 ER -