Genomic organization and molecular characterization of Clostridium difficile bacteriophage PhiCD119. Export

@article{citeulike:3469541, abstract = {In this study, we have isolated a temperate phage (PhiCD119) from a pathogenic Clostridium difficile strain and sequenced and annotated its genome. This virus has an icosahedral capsid and a contractile tail covered by a sheath and contains a double-stranded DNA genome. It belongs to the Myoviridae family of the tailed phages and the order Caudovirales. The genome was circularly permuted, with no physical ends detected by sequencing or restriction enzyme digestion analysis, and lacked a cos site. The DNA sequence of this phage consists of 53,325 bp, which carries 79 putative open reading frames (ORFs). A function could be assigned to 23 putative gene products, based upon bioinformatic analyses. The PhiCD119 genome is organized in a modular format, which includes modules for lysogeny, DNA replication, DNA packaging, structural proteins, and host cell lysis. The PhiCD119 attachment site attP lies in a noncoding region close to the putative integrase (int) gene. We have identified the phage integration site on the C. difficile chromosome (attB) located in a noncoding region just upstream of gene gltP, which encodes a carrier protein for glutamate and aspartate. This genetic analysis represents the first complete DNA sequence and annotation of a C. difficile phage.}, author = {Govind, Revathi and Fralick, Joe A. and Rolfe, Rial D.}, citeulike-article-id = {3469541}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JB.188.7.2568-2577.2006}, citeulike-linkout-1 = {http://jb.asm.org/cgi/content/abstract/188/7/2568}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16547044}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16547044}, day = {1}, doi = {10.1128/JB.188.7.2568-2577.2006}, issn = {0021-9193}, journal = {Journal of bacteriology}, keywords = {genome, lysogenic, phage}, month = {April}, number = {7}, pages = {2568--2577}, posted-at = {2008-10-31 14:53:38}, priority = {0}, title = {Genomic organization and molecular characterization of Clostridium difficile bacteriophage PhiCD119.}, url = {http://dx.doi.org/10.1128/JB.188.7.2568-2577.2006}, volume = {188}, year = {2006} }

TY - JOUR ID - citeulike:3469541 L3 - citeulike-article-id:3469541 N2 - In this study, we have isolated a temperate phage (PhiCD119) from a pathogenic Clostridium difficile strain and sequenced and annotated its genome. This virus has an icosahedral capsid and a contractile tail covered by a sheath and contains a double-stranded DNA genome. It belongs to the Myoviridae family of the tailed phages and the order Caudovirales. The genome was circularly permuted, with no physical ends detected by sequencing or restriction enzyme digestion analysis, and lacked a cos site. The DNA sequence of this phage consists of 53,325 bp, which carries 79 putative open reading frames (ORFs). A function could be assigned to 23 putative gene products, based upon bioinformatic analyses. The PhiCD119 genome is organized in a modular format, which includes modules for lysogeny, DNA replication, DNA packaging, structural proteins, and host cell lysis. The PhiCD119 attachment site attP lies in a noncoding region close to the putative integrase (int) gene. We have identified the phage integration site on the C. difficile chromosome (attB) located in a noncoding region just upstream of gene gltP, which encodes a carrier protein for glutamate and aspartate. This genetic analysis represents the first complete DNA sequence and annotation of a C. difficile phage. IS - 7 JF - Journal of bacteriology SN - 0021-9193 EP - 2577 TI - Genomic organization and molecular characterization of Clostridium difficile bacteriophage PhiCD119. VL - 188 SP - 2568 KW - genome KW - lysogenic KW - phage AU - Govind, Revathi AU - Fralick, Joe AU - Rolfe, Rial PY - 2006/04/01/ UR - http://dx.doi.org/10.1128/JB.188.7.2568-2577.2006 ER -