The complete genome sequence of Clostridium difficile phage phiC2 and comparisons to phiCD119 and inducible prophages of CD630

@article{citeulike:3484397, abstract = {The complete genomic sequence of a previously characterized temperate phage of Clostridium difficile,{phi} C2, is reported. The genome is 56 538 bp and organized into 84 putative ORFs in six functional modules. The head and tail structural proteins showed similarities to that of C. difficile phage{phi} CD119 and Streptococcus pneumoniae phage EJ-1, respectively. Homologues of structural and replication proteins were found in prophages 1 and 2 of the sequenced C. difficile CD630 genome. A putative holin appears unique to the C. difficile phages and was functional when expressed in Escherichia coli. Nucleotide sequence comparisons of{phi} C2 to{phi} CD119 and the CD630 prophage sequences showed relatedness between{phi} C2 and the prophages, but less so to{phi} CD119.{phi} C2 integrated into a gene encoding a putative transcriptional regulator of the gntR family.{phi} C2,{phi} CD119 and CD630 prophage 1 genomes had a Cdu1-attP-integrase arrangement, suggesting that the pathogenicity locus (PaLoc) of C. difficile, flanked by cdu1, has phage origins. The attP sequences of{phi} C2,{phi} CD119 and CD630 prophages were dissimilar. {phi}C2-related sequences were found in 84 \% of 37 clinical C. difficile isolates and typed reference strains. 10.1099/mic.0.2006/002436-0}, author = {Goh, Shan and Ong, Peh F. and Song, Keang P. and Riley, Thomas V. and Chang, Barbara J.}, citeulike-article-id = {3484397}, doi = {10.1099/mic.0.2006/002436-0}, journal = {Microbiology}, keywords = {genomics, lysogenic, prophage, temperate}, month = {March}, number = {3}, pages = {676--685}, posted-at = {2008-11-06 12:45:11}, priority = {3}, title = {The complete genome sequence of Clostridium difficile phage {phi}C2 and comparisons to {phi}CD119 and inducible prophages of CD630}, url = {http://dx.doi.org/10.1099/mic.0.2006/002436-0}, volume = {153}, year = {2007} }

TY - JOUR ID - citeulike:3484397 L3 - citeulike-article-id:3484397 N2 - The complete genomic sequence of a previously characterized temperate phage of Clostridium difficile,{phi} C2, is reported. The genome is 56 538 bp and organized into 84 putative ORFs in six functional modules. The head and tail structural proteins showed similarities to that of C. difficile phage{phi} CD119 and Streptococcus pneumoniae phage EJ-1, respectively. Homologues of structural and replication proteins were found in prophages 1 and 2 of the sequenced C. difficile CD630 genome. A putative holin appears unique to the C. difficile phages and was functional when expressed in Escherichia coli. Nucleotide sequence comparisons of{phi} C2 to{phi} CD119 and the CD630 prophage sequences showed relatedness between{phi} C2 and the prophages, but less so to{phi} CD119.{phi} C2 integrated into a gene encoding a putative transcriptional regulator of the gntR family.{phi} C2,{phi} CD119 and CD630 prophage 1 genomes had a Cdu1-attP-integrase arrangement, suggesting that the pathogenicity locus (PaLoc) of C. difficile, flanked by cdu1, has phage origins. The attP sequences of{phi} C2,{phi} CD119 and CD630 prophages were dissimilar. {phi}C2-related sequences were found in 84 % of 37 clinical C. difficile isolates and typed reference strains. 10.1099/mic.0.2006/002436-0 IS - 3 JF - Microbiology EP - 685 TI - The complete genome sequence of Clostridium difficile phage {phi}C2 and comparisons to {phi}CD119 and inducible prophages of CD630 VL - 153 SP - 676 KW - genomics KW - lysogenic KW - prophage KW - temperate AU - Goh, Shan AU - Ong, Peh AU - Song, Keang AU - Riley, Thomas AU - Chang, Barbara PY - 2007/03/01/ UR - http://dx.doi.org/10.1099/mic.0.2006/002436-0 ER -