PCR Targeted to the 16S-23S rRNA Gene Intergenic Spacer Region of Clostridium difficile and Construction of a Library Consisting of 116 Different PCR Ribotypes Export

@article{citeulike:3487504, abstract = {A reference library of types of Clostridium difficile has been constructed by PCR ribotyping isolates (n = 2,030) from environmental (n = 89), hospital (n = 1,386), community practitioner (n = 395), veterinary (n = 27), and reference (n = 133) sources. The library consists of 116 distinct types identified on the basis of differences in profiles generated with PCR primers designed to amplify the 16S-23S rRNA gene intergenic spacer region. Isolates from 55\% of infections in hospitals in the United Kingdom belonged to one ribotype (type 1), but this type was responsible for only 7.5\% of community infections.}, author = {Stubbs, Simon L. and Brazier, Jon S. and O'Neill, Gael L. and Duerden, Brian I.}, citeulike-article-id = {3487504}, citeulike-linkout-0 = {http://jcm.asm.org/cgi/content/abstract/37/2/461}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/9889244}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=9889244}, day = {1}, journal = {J. Clin. Microbiol.}, keywords = {pcr, ribotyping}, month = {February}, number = {2}, pages = {461--463}, posted-at = {2008-11-07 13:29:19}, priority = {2}, title = {PCR Targeted to the 16S-23S rRNA Gene Intergenic Spacer Region of Clostridium difficile and Construction of a Library Consisting of 116 Different PCR Ribotypes}, url = {http://jcm.asm.org/cgi/content/abstract/37/2/461}, volume = {37}, year = {1999} }

TY - JOUR ID - citeulike:3487504 L3 - citeulike-article-id:3487504 N2 - A reference library of types of Clostridium difficile has been constructed by PCR ribotyping isolates (n = 2,030) from environmental (n = 89), hospital (n = 1,386), community practitioner (n = 395), veterinary (n = 27), and reference (n = 133) sources. The library consists of 116 distinct types identified on the basis of differences in profiles generated with PCR primers designed to amplify the 16S-23S rRNA gene intergenic spacer region. Isolates from 55% of infections in hospitals in the United Kingdom belonged to one ribotype (type 1), but this type was responsible for only 7.5% of community infections. IS - 2 JF - J. Clin. Microbiol. EP - 463 TI - PCR Targeted to the 16S-23S rRNA Gene Intergenic Spacer Region of Clostridium difficile and Construction of a Library Consisting of 116 Different PCR Ribotypes VL - 37 SP - 461 KW - pcr KW - ribotyping AU - Stubbs, Simon AU - Brazier, Jon AU - O'Neill, Gael AU - Duerden, Brian PY - 1999/02/01/ UR - http://jcm.asm.org/cgi/content/abstract/37/2/461 ER -