Simple Method for Plating Escherichia coli Bacteriophages Forming Very Small Plaques or No Plaques under Standard Conditions Export

@article{citeulike:3680703, abstract = {The use of low concentrations (optimally 2.5 to 3.5 {micro}g/ml, depending on top agar thickness) of ampicillin in the bottom agar of the plate allows for formation of highly visible plaques of bacteriophages which otherwise form extremely small plaques or no plaques on Escherichia coli lawns. Using this method, we were able to obtain plaques of newly isolated bacteriophages, propagated after induction of prophages present in six E. coli O157:H- strains which did not form plaques when standard plating procedures were employed. 10.1128/AEM.00306-08}, author = {Los, Joanna M. and Golec, Piotr and Wegrzyn, Grzegorz and Wegrzyn, Alicja and Los, Marcin}, citeulike-article-id = {3680703}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/AEM.00306-08}, citeulike-linkout-1 = {http://aem.asm.org/cgi/content/abstract/74/16/5113}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18586961}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18586961}, day = {15}, doi = {10.1128/AEM.00306-08}, journal = {Appl. Environ. Microbiol.}, keywords = {isolation, method, plating}, month = {August}, number = {16}, pages = {5113--5120}, posted-at = {2008-11-24 11:30:40}, priority = {2}, title = {Simple Method for Plating Escherichia coli Bacteriophages Forming Very Small Plaques or No Plaques under Standard Conditions}, url = {http://dx.doi.org/10.1128/AEM.00306-08}, volume = {74}, year = {2008} }

TY - JOUR ID - citeulike:3680703 L3 - citeulike-article-id:3680703 N2 - The use of low concentrations (optimally 2.5 to 3.5 {micro}g/ml, depending on top agar thickness) of ampicillin in the bottom agar of the plate allows for formation of highly visible plaques of bacteriophages which otherwise form extremely small plaques or no plaques on Escherichia coli lawns. Using this method, we were able to obtain plaques of newly isolated bacteriophages, propagated after induction of prophages present in six E. coli O157:H- strains which did not form plaques when standard plating procedures were employed. 10.1128/AEM.00306-08 IS - 16 JF - Appl. Environ. Microbiol. EP - 5120 TI - Simple Method for Plating Escherichia coli Bacteriophages Forming Very Small Plaques or No Plaques under Standard Conditions VL - 74 SP - 5113 KW - isolation KW - method KW - plating AU - Los, Joanna AU - Golec, Piotr AU - Wegrzyn, Grzegorz AU - Wegrzyn, Alicja AU - Los, Marcin PY - 2008/08/15/ UR - http://dx.doi.org/10.1128/AEM.00306-08 ER -