Experimental Protection of Mice against Lethal Staphylococcus aureus Infection by Novel Bacteriophage φMR11 TeX Export

@article{citeulike:3801377, abstract = {doi: 10.1086/374001 PMID: 12599078 The protective effects of bacteriophages were assessed against experimental Staphylococcus aureus infection in mice. Of the S. aureus phages isolated in the study, φMR11 was representatively used for all testing, because its host range was the most broad and it carries no genes for known toxins or antibiotic resistance. Intraperitoneal injections ( \$8\times 10^{8}\$ cells) of S. aureus, including methicillin-resistant bacteria, caused bacteremia and eventual death in mice. In contrast, subsequent intraperitoneal administration of purified φMR11 (MOI 0.1) suppressed S. aureus-induced lethality. This lifesaving effect coincided with the rapid appearance of φMR11 in the circulation, which remained at substantial levels until the bacteria were eradicated. Inoculation with high-dose φMR11 alone produced no adverse effects attributable to the phage. These results uphold the efficacy of phage therapy against pernicious S. aureus infections in humans and suggest that φMR11 may be a potential prototype for gene-modified, advanced therapeutic S. aureus phages.}, author = {Matsuzaki, Shigenobu and Yasuda, Masaharu and Nishikawa, Hiroshi and Kuroda, Masayuki and Ujihara, Takako and Shuin, Taro and Shen, Yuan and Jin, Zhe and Fujimoto, Shigeyoshi and Nasimuzzaman, M. D. and Wakiguchi, Hiroshi and Sugihara, Shigeyoshi and Sugiura, Tetsuro and Koda, Shigeki and Muraoka, Asako and Imai, Shosuke}, citeulike-article-id = {3801377}, citeulike-linkout-0 = {http://dx.doi.org/10.1086/374001}, citeulike-linkout-1 = {http://www.journals.uchicago.edu/doi/abs/10.1086/374001}, day = {15}, doi = {10.1086/374001}, journal = {The Journal of Infectious Diseases}, keywords = {mice, staphylococcus}, month = {February}, number = {4}, pages = {613--624}, posted-at = {2008-12-17 12:40:20}, priority = {2}, title = {Experimental Protection of Mice against Lethal Staphylococcus aureus Infection by Novel Bacteriophage φMR11}, url = {http://dx.doi.org/10.1086/374001}, volume = {187}, year = {2003} }

TY - JOUR ID - citeulike:3801377 L3 - citeulike-article-id:3801377 N2 - doi: 10.1086/374001 PMID: 12599078 The protective effects of bacteriophages were assessed against experimental Staphylococcus aureus infection in mice. Of the S. aureus phages isolated in the study, φMR11 was representatively used for all testing, because its host range was the most broad and it carries no genes for known toxins or antibiotic resistance. Intraperitoneal injections ( $8\times 10^{8}$ cells) of S. aureus, including methicillin-resistant bacteria, caused bacteremia and eventual death in mice. In contrast, subsequent intraperitoneal administration of purified φMR11 (MOI 0.1) suppressed S. aureus-induced lethality. This lifesaving effect coincided with the rapid appearance of φMR11 in the circulation, which remained at substantial levels until the bacteria were eradicated. Inoculation with high-dose φMR11 alone produced no adverse effects attributable to the phage. These results uphold the efficacy of phage therapy against pernicious S. aureus infections in humans and suggest that φMR11 may be a potential prototype for gene-modified, advanced therapeutic S. aureus phages. IS - 4 JF - The Journal of Infectious Diseases EP - 624 TI - Experimental Protection of Mice against Lethal Staphylococcus aureus Infection by Novel Bacteriophage φMR11 VL - 187 SP - 613 KW - mice KW - staphylococcus AU - Matsuzaki, Shigenobu AU - Yasuda, Masaharu AU - Nishikawa, Hiroshi AU - Kuroda, Masayuki AU - Ujihara, Takako AU - Shuin, Taro AU - Shen, Yuan AU - Jin, Zhe AU - Fujimoto, Shigeyoshi AU - Nasimuzzaman, MD AU - Wakiguchi, Hiroshi AU - Sugihara, Shigeyoshi AU - Sugiura, Tetsuro AU - Koda, Shigeki AU - Muraoka, Asako AU - Imai, Shosuke PY - 2003/02/15/ UR - http://dx.doi.org/10.1086/374001 ER -