CYP17 and SULT1A1 gene polymorphisms in Indian breast cancer. Export

@article{citeulike:4750652, abstract = {BACKGROUND: Breast cancer is the most common cancer among women worldwide. Life-time exposure to steroid hormones, especially estrogen, is a major risk factor for breast cancer. Functional polymorphisms in genes encoding steroid metabolizing enzymes may thus be important as biomarkers of individual susceptibility to breast cancer. The CYP17 and SULT1A1 genes encode for two enzymes involved in hormone biosynthesis and metabolism. Single nucleotide polymorphisms of these genes may result in inter-individual variability in steroid hormone biosynthesis and metabolism thus influence the development of breast cancer. METHODS: We tested this hypothesis by conducting a case - control study on a group of 140 breast cancer cases and 140 healthy age-matched controls. Analysis of CYP17 and SULT1A1 genotypes were done by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). RESULTS: The genetic polymorphisms of the estrogen-related genes SULT1A1(OR=2.5, 95\% CI=1.28-4.98) and CYP17 (OR=4.1, 95= CI=1.78-9.63) were associated with an increased risk of breast cancer among postmenopausal women. Our data also showed evidence for the genetic regulation of serum 17 beta estradiol (E2) levels as measured by ELISA among the premenopausal women with a significant increase in the serum E2 level for the CYP17 A2 variants. CONCLUSION: These results suggest that both CYP17 and SULT1A1 genotypes could be important determinants of breast cancer risk in Indian women and may help in early identification of high risk subjects. Such genotype analysis resulting in a high-risk profile holds considerable promise for individualizing screening, diagnosis and therapeutic intervention in breast cancer.}, author = {Chacko, Priya and Rajan, Balakrishnan and Mathew, Beela Sarah S. and Joseph, Thomas and Pillai, M. Radhakrishnai}, citeulike-article-id = {4750652}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/15604994}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=15604994}, issn = {1340-6868}, journal = {Breast cancer (Tokyo, Japan)}, keywords = {breastcancer, cyp17, polymorphism, sult1a1}, number = {4}, pages = {380--388}, posted-at = {2009-06-05 12:54:24}, priority = {2}, title = {CYP17 and SULT1A1 gene polymorphisms in Indian breast cancer.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/15604994}, volume = {11}, year = {2004} }

TY - JOUR ID - citeulike:4750652 L3 - citeulike-article-id:4750652 N2 - BACKGROUND: Breast cancer is the most common cancer among women worldwide. Life-time exposure to steroid hormones, especially estrogen, is a major risk factor for breast cancer. Functional polymorphisms in genes encoding steroid metabolizing enzymes may thus be important as biomarkers of individual susceptibility to breast cancer. The CYP17 and SULT1A1 genes encode for two enzymes involved in hormone biosynthesis and metabolism. Single nucleotide polymorphisms of these genes may result in inter-individual variability in steroid hormone biosynthesis and metabolism thus influence the development of breast cancer. METHODS: We tested this hypothesis by conducting a case - control study on a group of 140 breast cancer cases and 140 healthy age-matched controls. Analysis of CYP17 and SULT1A1 genotypes were done by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). RESULTS: The genetic polymorphisms of the estrogen-related genes SULT1A1(OR=2.5, 95% CI=1.28-4.98) and CYP17 (OR=4.1, 95= CI=1.78-9.63) were associated with an increased risk of breast cancer among postmenopausal women. Our data also showed evidence for the genetic regulation of serum 17 beta estradiol (E2) levels as measured by ELISA among the premenopausal women with a significant increase in the serum E2 level for the CYP17 A2 variants. CONCLUSION: These results suggest that both CYP17 and SULT1A1 genotypes could be important determinants of breast cancer risk in Indian women and may help in early identification of high risk subjects. Such genotype analysis resulting in a high-risk profile holds considerable promise for individualizing screening, diagnosis and therapeutic intervention in breast cancer. IS - 4 JF - Breast cancer (Tokyo, Japan) SN - 1340-6868 EP - 388 TI - CYP17 and SULT1A1 gene polymorphisms in Indian breast cancer. VL - 11 SP - 380 KW - breastcancer KW - cyp17 KW - polymorphism KW - sult1a1 AU - Chacko, Priya AU - Rajan, Balakrishnan AU - Mathew, Beela Sarah AU - Joseph, Thomas AU - Pillai, Radhakrishnai PY - 2004/// UR - http://view.ncbi.nlm.nih.gov/pubmed/15604994 ER -