Pollen-stigma interactions in Brassica oleracea; a new pollen germination medium and its use in elucidating the mechanism of self incompatibility Export

@article{citeulike:3735568, abstract = {A simple medium is described which supports the germination of Brassica pollen in vitro. The method is completely reliable and the percentage germination achieved is comparable to that found on a compatible stigma. The pollen tubes produced attain lengths equal to those growing in the style and exhibit similar growth kinetics. The major difference between this medium and other less-reliable media is the addition of 1 mM Tris. The efficacy of Tris is shown to be due in part to the establishment of an optimum pH but an ” amine effect” is also identified. The optimum pH for growth in vitro may be different from that encountered on the stigma. Atmospheric concentration of CO2 and pollen population density are also shown to have pronounced effects on germination in vitro.}, author = {Roberts, I. N. and Gaude, T. C. and Harrod, G. and Dickinson, H. G.}, citeulike-article-id = {3735568}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/BF00308074}, citeulike-linkout-1 = {http://www.springerlink.com/content/w30p1j10101586v7}, day = {1}, doi = {10.1007/BF00308074}, journal = {TAG Theoretical and Applied Genetics}, keywords = {pollen}, month = {May}, number = {3}, pages = {231--238}, posted-at = {2008-12-02 14:50:45}, priority = {2}, title = {Pollen-stigma interactions in Brassica oleracea; a new pollen germination medium and its use in elucidating the mechanism of self incompatibility}, url = {http://dx.doi.org/10.1007/BF00308074}, volume = {65}, year = {1983} }

TY - JOUR ID - citeulike:3735568 L3 - citeulike-article-id:3735568 N2 - A simple medium is described which supports the germination of Brassica pollen in vitro. The method is completely reliable and the percentage germination achieved is comparable to that found on a compatible stigma. The pollen tubes produced attain lengths equal to those growing in the style and exhibit similar growth kinetics. The major difference between this medium and other less-reliable media is the addition of 1 mM Tris. The efficacy of Tris is shown to be due in part to the establishment of an optimum pH but an “amine effect” is also identified. The optimum pH for growth in vitro may be different from that encountered on the stigma. Atmospheric concentration of CO2 and pollen population density are also shown to have pronounced effects on germination in vitro. IS - 3 JF - TAG Theoretical and Applied Genetics EP - 238 TI - Pollen-stigma interactions in Brassica oleracea; a new pollen germination medium and its use in elucidating the mechanism of self incompatibility VL - 65 SP - 231 KW - pollen AU - Roberts, IN AU - Gaude, TC AU - Harrod, G AU - Dickinson, HG PY - 1983/05/01/ UR - http://dx.doi.org/10.1007/BF00308074 ER -