Identification of a target RNA motif for RNA-binding protein HuR Export

@article{citeulike:5365270, abstract = {10.1073/pnas.0306453101 HuR, a protein that binds to specific mRNA subsets, is increasingly recognized as a pivotal posttranscriptional regulator of gene expression. Here, HuR was immunoprecipitated under conditions that preserved HuR-RNA interactions, and HuR-bound target mRNAs were identified by cDNA array hybridization. Analysis of primary sequences and secondary structures shared among HuR targets led to the identification of a 17- to 20-base-long RNA motif rich in uracils. This HuR motif was found in almost all mRNAs previously reported to be HuR targets, was located preferentially within 3\^{a}€² untranslated regions of all unigene transcripts examined, and was conserved in >50\% of human and mouse homologous genes. Importantly, the HuR motif allowed the successful prediction and subsequent validation of novel HuR targets from gene databases. This study describes an HuR target RNA motif and presents a general strategy for identifying target motifs for RNA-binding proteins.}, author = {de Silanes, Isabel L. and Zhan, Ming and Lal, Ashish and Yang, Xiaoling and Gorospe, Myriam}, citeulike-article-id = {5365270}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0306453101}, citeulike-linkout-1 = {http://www.pnas.org/content/101/9/2987.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/101/9/2987.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/14981256}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=14981256}, day = {2}, doi = {10.1073/pnas.0306453101}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {hur, motif, posttranscriptional\_regulator, rna\_binding\_protein}, month = {March}, number = {9}, pages = {2987--2992}, posted-at = {2009-11-06 14:55:14}, priority = {2}, title = {Identification of a target RNA motif for RNA-binding protein HuR}, url = {http://dx.doi.org/10.1073/pnas.0306453101}, volume = {101}, year = {2004} }

TY - JOUR ID - citeulike:5365270 L3 - citeulike-article-id:5365270 N2 - 10.1073/pnas.0306453101 HuR, a protein that binds to specific mRNA subsets, is increasingly recognized as a pivotal posttranscriptional regulator of gene expression. Here, HuR was immunoprecipitated under conditions that preserved HuR-RNA interactions, and HuR-bound target mRNAs were identified by cDNA array hybridization. Analysis of primary sequences and secondary structures shared among HuR targets led to the identification of a 17- to 20-base-long RNA motif rich in uracils. This HuR motif was found in almost all mRNAs previously reported to be HuR targets, was located preferentially within 3′ untranslated regions of all unigene transcripts examined, and was conserved in >50% of human and mouse homologous genes. Importantly, the HuR motif allowed the successful prediction and subsequent validation of novel HuR targets from gene databases. This study describes an HuR target RNA motif and presents a general strategy for identifying target motifs for RNA-binding proteins. IS - 9 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 EP - 2992 TI - Identification of a target RNA motif for RNA-binding protein HuR VL - 101 SP - 2987 KW - hur KW - motif KW - posttranscriptional_regulator KW - rna_binding_protein AU - de Silanes, Isabel AU - Zhan, Ming AU - Lal, Ashish AU - Yang, Xiaoling AU - Gorospe, Myriam PY - 2004/03/02/ UR - http://dx.doi.org/10.1073/pnas.0306453101 ER -