RAS Is Regulated by the let-7 MicroRNA Family Export

@article{citeulike:550511, abstract = {MicroRNAs (miRNAs) are regulatory RNAs found in multicellular eukaryotes, including humans, where they are implicated in cancer. The let-7 miRNA times seam cell terminal differentiation in C. elegans. Here we show that the let-7 family negatively regulates let-60/RAS. Loss of let-60/RAS suppresses let-7, and the let-60/RAS 3'UTR contains multiple let-7 complementary sites (LCSs), restricting reporter gene expression in a let-7-dependent manner. mir-84, a let-7 family member, is largely absent in vulval precursor cell P6.p at the time that let-60/RAS specifies the 1 degrees vulval fate in that cell, and mir-84 overexpression suppresses the multivulva phenotype of activating let-60/RAS mutations. The 3'UTRs of the human RAS genes contain multiple LCSs, allowing let-7 to regulate RAS expression. let-7 expression is lower in lung tumors than in normal lung tissue, while RAS protein is significantly higher in lung tumors, providing a possible mechanism for let-7 in cancer.}, address = {Department of Molecular, Cellular and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520, USA.}, author = {Johnson, Steven M. and Grosshans, Helge and Shingara, Jaclyn and Byrom, Mike and Jarvis, Rich and Cheng, Angie and Labourier, Emmanuel and Reinert, Kristy L. and Brown, David and Slack, Frank J.}, citeulike-article-id = {550511}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2005.01.014}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0092867405000887}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15766527}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15766527}, citeulike-linkout-4 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00025025}, day = {11}, doi = {10.1016/j.cell.2005.01.014}, issn = {00928674}, journal = {Cell}, keywords = {c\_elegans, let-7, microrna, ras, ras\_pathway}, month = {March}, number = {5}, pages = {635--647}, posted-at = {2009-11-06 13:47:02}, priority = {2}, title = {RAS Is Regulated by the let-7 MicroRNA Family}, url = {http://dx.doi.org/10.1016/j.cell.2005.01.014}, volume = {120}, year = {2005} }

TY - JOUR ID - citeulike:550511 L3 - citeulike-article-id:550511 N2 - MicroRNAs (miRNAs) are regulatory RNAs found in multicellular eukaryotes, including humans, where they are implicated in cancer. The let-7 miRNA times seam cell terminal differentiation in C. elegans. Here we show that the let-7 family negatively regulates let-60/RAS. Loss of let-60/RAS suppresses let-7, and the let-60/RAS 3'UTR contains multiple let-7 complementary sites (LCSs), restricting reporter gene expression in a let-7-dependent manner. mir-84, a let-7 family member, is largely absent in vulval precursor cell P6.p at the time that let-60/RAS specifies the 1 degrees vulval fate in that cell, and mir-84 overexpression suppresses the multivulva phenotype of activating let-60/RAS mutations. The 3'UTRs of the human RAS genes contain multiple LCSs, allowing let-7 to regulate RAS expression. let-7 expression is lower in lung tumors than in normal lung tissue, while RAS protein is significantly higher in lung tumors, providing a possible mechanism for let-7 in cancer. IS - 5 JF - Cell SN - 00928674 AD - Department of Molecular, Cellular and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520, USA. EP - 647 TI - RAS Is Regulated by the let-7 MicroRNA Family VL - 120 SP - 635 KW - c_elegans KW - let-7 KW - microrna KW - ras KW - ras_pathway AU - Johnson, Steven AU - Grosshans, Helge AU - Shingara, Jaclyn AU - Byrom, Mike AU - Jarvis, Rich AU - Cheng, Angie AU - Labourier, Emmanuel AU - Reinert, Kristy AU - Brown, David AU - Slack, Frank PY - 2005/03/11/ UR - http://dx.doi.org/10.1016/j.cell.2005.01.014 ER -