Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells Export

@article{citeulike:5898959, abstract = {The transcription factors OCT4, SOX2, and NANOG have essential roles in early development and are required for the propagation of undifferentiated embryonic stem (ES) cells in culture. To gain insights into transcriptional regulation of human ES cells, we have identified OCT4, SOX2, and NANOG target genes using genome-scale location analysis. We found, surprisingly, that OCT4, SOX2, and NANOG co-occupy a substantial portion of their target genes. These target genes frequently encode transcription factors, many of which are developmentally important homeodomain proteins. Our data also indicate that OCT4, SOX2, and NANOG collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops. These results provide new insights into the transcriptional regulation of stem cells and reveal how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal.}, author = {Boyer, Laurie A. and Lee, Tong I. and Cole, Megan F. and Johnstone, Sarah E. and Levine, Stuart S. and Zucker, Jacob P. and Guenther, Matthew G. and Kumar, Roshan M. and Murray, Heather L. and Jenner, Richard G. and Gifford, David K. and Melton, Douglas A. and Jaenisch, Rudolf and Young, Richard A.}, citeulike-article-id = {5898959}, citeulike-linkout-0 = {http://www.cell.com//abstract/S0092-8674(05)00825-1}, day = {23}, keywords = {binding\_motif, embryonic, embryonic\_stem\_cell, nanog, oct4, sox2, stem, tf}, month = {September}, number = {6}, pages = {947--956}, posted-at = {2009-10-06 15:29:21}, priority = {2}, title = {Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells}, url = {http://www.cell.com//abstract/S0092-8674(05)00825-1}, volume = {122}, year = {2005} }

TY - JOUR ID - citeulike:5898959 L3 - citeulike-article-id:5898959 N2 - The transcription factors OCT4, SOX2, and NANOG have essential roles in early development and are required for the propagation of undifferentiated embryonic stem (ES) cells in culture. To gain insights into transcriptional regulation of human ES cells, we have identified OCT4, SOX2, and NANOG target genes using genome-scale location analysis. We found, surprisingly, that OCT4, SOX2, and NANOG co-occupy a substantial portion of their target genes. These target genes frequently encode transcription factors, many of which are developmentally important homeodomain proteins. Our data also indicate that OCT4, SOX2, and NANOG collaborate to form regulatory circuitry consisting of autoregulatory and feedforward loops. These results provide new insights into the transcriptional regulation of stem cells and reveal how OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal. IS - 6 EP - 956 TI - Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells VL - 122 SP - 947 KW - binding_motif KW - embryonic KW - embryonic_stem_cell KW - nanog KW - oct4 KW - sox2 KW - stem KW - tf AU - Boyer, Laurie AU - Lee, Tong AU - Cole, Megan AU - Johnstone, Sarah AU - Levine, Stuart AU - Zucker, Jacob AU - Guenther, Matthew AU - Kumar, Roshan AU - Murray, Heather AU - Jenner, Richard AU - Gifford, David AU - Melton, Douglas AU - Jaenisch, Rudolf AU - Young, Richard PY - 2005/09/23/ UR - http://www.cell.com//abstract/S0092-8674(05)00825-1 ER -