The RNA-Binding Protein HuR Promotes Cell Migration and Cell Invasion by Stabilizing the beta-actin mRNA in a U-Rich-Element-Dependent Manner Export

@article{citeulike:6081050, abstract = {A high expression level of the {beta}-actin protein is required for important biological mechanisms, such as maintaining cell shape, growth, and motility. Although the elevated cellular level of the {beta}-actin protein is directly linked to the long half-life of its mRNA, the molecular mechanisms responsible for this effect are unknown. Here we show that the RNA-binding protein HuR stabilizes the {beta}-actin mRNA by associating with a uridine-rich element within its 3' untranslated region. Using RNA interference to knock down the expression of HuR in HeLa cells, we demonstrate that HuR plays an important role in the stabilization but not in the nuclear/cytoplasmic distribution of the {beta}-actin mRNA. HuR depletion in HeLa cells alters key {beta}-actin-based cytoskeleton functions, such as cell adhesion, migration, and invasion, and these defects correlate with a loss of the actin stress fiber network. Together our data establish that the posttranscriptional event involving HuR-mediated {beta}-actin mRNA stabilization could be a part of the regulatory mechanisms responsible for maintaining cell integrity, which is a prerequisite for avoiding transformation and tumor formation. 10.1128/MCB.00113-07}, author = {Dormoy-Raclet, Virginie and Menard, Isabelle and Clair, Eveline and Kurban, Ghada and Mazroui, Rachid and Di Marco, Sergio and von Roretz, Christopher and Pause, Arnim and Gallouzi, Imed-Eddine}, citeulike-article-id = {6081050}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/MCB.00113-07}, citeulike-linkout-1 = {http://mcb.asm.org/content/27/15/5365.abstract}, citeulike-linkout-2 = {http://mcb.asm.org/content/27/15/5365.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/17548472}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=17548472}, day = {1}, doi = {10.1128/MCB.00113-07}, journal = {Mol. Cell. Biol.}, keywords = {hur, rna\_binding\_protein}, month = {August}, number = {15}, pages = {5365--5380}, posted-at = {2009-11-06 15:15:47}, priority = {2}, title = {The RNA-Binding Protein HuR Promotes Cell Migration and Cell Invasion by Stabilizing the {beta}-actin mRNA in a U-Rich-Element-Dependent Manner}, url = {http://dx.doi.org/10.1128/MCB.00113-07}, volume = {27}, year = {2007} }

TY - JOUR ID - citeulike:6081050 L3 - citeulike-article-id:6081050 N2 - A high expression level of the {beta}-actin protein is required for important biological mechanisms, such as maintaining cell shape, growth, and motility. Although the elevated cellular level of the {beta}-actin protein is directly linked to the long half-life of its mRNA, the molecular mechanisms responsible for this effect are unknown. Here we show that the RNA-binding protein HuR stabilizes the {beta}-actin mRNA by associating with a uridine-rich element within its 3' untranslated region. Using RNA interference to knock down the expression of HuR in HeLa cells, we demonstrate that HuR plays an important role in the stabilization but not in the nuclear/cytoplasmic distribution of the {beta}-actin mRNA. HuR depletion in HeLa cells alters key {beta}-actin-based cytoskeleton functions, such as cell adhesion, migration, and invasion, and these defects correlate with a loss of the actin stress fiber network. Together our data establish that the posttranscriptional event involving HuR-mediated {beta}-actin mRNA stabilization could be a part of the regulatory mechanisms responsible for maintaining cell integrity, which is a prerequisite for avoiding transformation and tumor formation. 10.1128/MCB.00113-07 IS - 15 JF - Mol. Cell. Biol. EP - 5380 TI - The RNA-Binding Protein HuR Promotes Cell Migration and Cell Invasion by Stabilizing the {beta}-actin mRNA in a U-Rich-Element-Dependent Manner VL - 27 SP - 5365 KW - hur KW - rna_binding_protein AU - Dormoy-Raclet, Virginie AU - Menard, Isabelle AU - Clair, Eveline AU - Kurban, Ghada AU - Mazroui, Rachid AU - Di Marco, Sergio AU - von Roretz, Christopher AU - Pause, Arnim AU - Gallouzi, Imed-Eddine PY - 2007/08/01/ UR - http://dx.doi.org/10.1128/MCB.00113-07 ER -