Comparative Bacterial Proteomics: Analysis of the Core Genome Concept

@article{citeulike:3043831, abstract = {While comparative bacterial genomic studies commonly predict a set of genes indicative of common ancestry, experimental validation of the existence of this core genome requires extensive measurement and is typically not undertaken. Enabled by an extensive proteome database developed over six years, we have experimentally verified the expression of proteins predicted from genomic ortholog comparisons among 17 environmental and pathogenic bacteria. More exclusive relationships were observed among the expressed protein content of phenotypically related bacteria, which is indicative of the specific lifestyles associated with these organisms. Although genomic studies can establish relative orthologous relationships among a set of bacteria and propose a set of ancestral genes, our proteomics study establishes expressed lifestyle differences among conserved genes and proposes a set of expressed ancestral traits.}, author = {Callister, Stephen J. and Mccue, Lee A. and Turse, Joshua E. and Monroe, Matthew E. and Auberry, Kenneth J. and Smith, Richard D. and Adkins, Joshua N. and Lipton, Mary S.}, citeulike-article-id = {3043831}, doi = {10.1371/journal.pone.0001542}, journal = {PLoS ONE}, keywords = {bacteria, core, genome, proteome}, month = {February}, number = {2}, pages = {e1542+}, posted-at = {2008-11-20 14:02:10}, priority = {2}, publisher = {Public Library of Science}, title = {Comparative Bacterial Proteomics: Analysis of the Core Genome Concept}, url = {http://dx.doi.org/10.1371/journal.pone.0001542}, volume = {3}, year = {2008} }

TY - JOUR ID - citeulike:3043831 L3 - citeulike-article-id:3043831 N2 - While comparative bacterial genomic studies commonly predict a set of genes indicative of common ancestry, experimental validation of the existence of this core genome requires extensive measurement and is typically not undertaken. Enabled by an extensive proteome database developed over six years, we have experimentally verified the expression of proteins predicted from genomic ortholog comparisons among 17 environmental and pathogenic bacteria. More exclusive relationships were observed among the expressed protein content of phenotypically related bacteria, which is indicative of the specific lifestyles associated with these organisms. Although genomic studies can establish relative orthologous relationships among a set of bacteria and propose a set of ancestral genes, our proteomics study establishes expressed lifestyle differences among conserved genes and proposes a set of expressed ancestral traits. IS - 2 JF - PLoS ONE TI - Comparative Bacterial Proteomics: Analysis of the Core Genome Concept PB - Public Library of Science VL - 3 SP - e1542 KW - bacteria KW - core KW - genome KW - proteome AU - Callister, Stephen AU - Mccue, Lee AU - Turse, Joshua AU - Monroe, Matthew AU - Auberry, Kenneth AU - Smith, Richard AU - Adkins, Joshua AU - Lipton, Mary PY - 2008/02/06/ UR - http://dx.doi.org/10.1371/journal.pone.0001542 ER -