Site-specific labeling of proteins with small molecules in live cells Export

@article{citeulike:115121, abstract = {The principal bottleneck for the utilization of small-molecule probes in live cells is the shortage of methodologies for targeting them with very high specificity to biological molecules or compartments of interest. Recently developed methods for labeling proteins with small-molecule probes in cells employ special protein or peptide handles that recruit small-molecule ligands, harness enzymes to catalyze small-molecule conjugation or hijack the cell's protein translation machinery.}, address = {Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.}, author = {Chen, I. and Ting, A.}, citeulike-article-id = {115121}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.copbio.2004.12.003}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0958166904001685}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15722013}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15722013}, citeulike-linkout-4 = {http://www.sciencedirect.com/science/article/B6VRV-4F37MCT-1/2/0f11a6c0d2c0148c778f491eb89ab6e5}, doi = {10.1016/j.copbio.2004.12.003}, issn = {09581669}, journal = {Current Opinion in Biotechnology}, keywords = {fluorescence, gfp, microscopy, tetracysteine, vivo}, month = {February}, number = {1}, pages = {35--40}, posted-at = {2008-10-16 11:19:56}, priority = {2}, title = {Site-specific labeling of proteins with small molecules in live cells}, url = {http://dx.doi.org/10.1016/j.copbio.2004.12.003}, volume = {16}, year = {2005} }

TY - JOUR ID - citeulike:115121 L3 - citeulike-article-id:115121 N2 - The principal bottleneck for the utilization of small-molecule probes in live cells is the shortage of methodologies for targeting them with very high specificity to biological molecules or compartments of interest. Recently developed methods for labeling proteins with small-molecule probes in cells employ special protein or peptide handles that recruit small-molecule ligands, harness enzymes to catalyze small-molecule conjugation or hijack the cell's protein translation machinery. IS - 1 JF - Current Opinion in Biotechnology SN - 09581669 AD - Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. EP - 40 TI - Site-specific labeling of proteins with small molecules in live cells VL - 16 SP - 35 KW - fluorescence KW - gfp KW - microscopy KW - tetracysteine KW - vivo AU - Chen, I AU - Ting, A PY - 2005/02// UR - http://dx.doi.org/10.1016/j.copbio.2004.12.003 ER -