Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy Export

@article{citeulike:3169223, abstract = {10.1073/pnas.0608509103 Combinations of molecular tags visible in light and electron microscopes become particularly advantageous in the analysis of dynamic cellular components like the Golgi apparatus. This organelle disassembles at the onset of mitosis and, after a sequence of poorly understood events, reassembles after cytokinesis. The precise location of Golgi membranes and resident proteins during mitosis remains unclear, partly due to limitations of molecular markers and the resolution of light microscopy. We generated a fusion consisting of the first 117 residues of α-mannosidase II tagged with a fluorescent protein and a tetracysteine motif. The mannosidase component guarantees docking into the Golgi membrane, with the tags exposed in the lumen. The fluorescent protein is optically visible without further treatment, whereas the tetracysteine tag can be reduced acutely with a membrane-permeant phosphine, labeled with ReAsH, monitored in the light microscope, and used to trigger the photoconversion of diaminobenzidine, allowing 4D optical recording on live cells and correlated ultrastructural analysis by electron microscopy. These methods reveal that Golgi reassembly is preceded by the formation of four colinear clusters at telophase, two per daughter cell. Within each daughter, the smaller cluster near the midbody gradually migrates to rejoin the major cluster on the far side of the nucleus and asymmetrically reconstitutes a single Golgi apparatus, first in one daughter cell and then in the other. Our studies provide previously undescribed insights into Golgi disassociation and reassembly during mitosis and offer a powerful approach to follow recombinant protein distribution in 4D imaging and correlated high-resolution analysis.}, author = {Gaietta, Guido M. and Giepmans, Ben N. and Deerinck, Thomas J. and Smith, Bryan W. and Ngan, Lucy and Llopis, Juan and Adams, Stephen R. and Tsien, Roger Y. and Ellisman, Mark H.}, citeulike-article-id = {3169223}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0608509103}, citeulike-linkout-1 = {http://www.pnas.org/content/103/47/17777.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/103/47/17777.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/17101980}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=17101980}, day = {21}, doi = {10.1073/pnas.0608509103}, journal = {Proceedings of the National Academy of Sciences}, keywords = {clem, fret, gfp, golgi, grab, mitosis, reash, reducing-environment, tetracysteine}, month = {November}, number = {47}, pages = {17777--17782}, posted-at = {2008-08-28 10:32:29}, priority = {2}, title = {Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy}, url = {http://dx.doi.org/10.1073/pnas.0608509103}, volume = {103}, year = {2006} }

TY - JOUR ID - citeulike:3169223 L3 - citeulike-article-id:3169223 N2 - 10.1073/pnas.0608509103 Combinations of molecular tags visible in light and electron microscopes become particularly advantageous in the analysis of dynamic cellular components like the Golgi apparatus. This organelle disassembles at the onset of mitosis and, after a sequence of poorly understood events, reassembles after cytokinesis. The precise location of Golgi membranes and resident proteins during mitosis remains unclear, partly due to limitations of molecular markers and the resolution of light microscopy. We generated a fusion consisting of the first 117 residues of α-mannosidase II tagged with a fluorescent protein and a tetracysteine motif. The mannosidase component guarantees docking into the Golgi membrane, with the tags exposed in the lumen. The fluorescent protein is optically visible without further treatment, whereas the tetracysteine tag can be reduced acutely with a membrane-permeant phosphine, labeled with ReAsH, monitored in the light microscope, and used to trigger the photoconversion of diaminobenzidine, allowing 4D optical recording on live cells and correlated ultrastructural analysis by electron microscopy. These methods reveal that Golgi reassembly is preceded by the formation of four colinear clusters at telophase, two per daughter cell. Within each daughter, the smaller cluster near the midbody gradually migrates to rejoin the major cluster on the far side of the nucleus and asymmetrically reconstitutes a single Golgi apparatus, first in one daughter cell and then in the other. Our studies provide previously undescribed insights into Golgi disassociation and reassembly during mitosis and offer a powerful approach to follow recombinant protein distribution in 4D imaging and correlated high-resolution analysis. IS - 47 JF - Proceedings of the National Academy of Sciences EP - 17782 TI - Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy VL - 103 SP - 17777 KW - clem KW - fret KW - gfp KW - golgi KW - grab KW - mitosis KW - reash KW - reducing-environment KW - tetracysteine AU - Gaietta, Guido AU - Giepmans, Ben AU - Deerinck, Thomas AU - Smith, Bryan AU - Ngan, Lucy AU - Llopis, Juan AU - Adams, Stephen AU - Tsien, Roger AU - Ellisman, Mark PY - 2006/11/21/ UR - http://dx.doi.org/10.1073/pnas.0608509103 ER -