Visualizing intracellular events in vivo by combined video fluorescence and 3-D electron microscopy. Export

@article{citeulike:3174917, abstract = {The combination of the capability of in vivo fluorescence video microscopy with the power of resolution of electron microscopy (EM) has been described. This approach is based on such an association of two techniques. An individual intracellular structure can be monitored in vivo, typically through the use of markers fused with green fluorescent protein (GFP), and a "snapshot" of its three-dimensional (3-D) ultrastructure and especially tomographic reconstruction can then be taken at any chosen time during its life cycle. The pitfalls and potential of this approach are discussed.}, address = {Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), Italy.}, author = {Mironov, A. A. and Beznoussenko, G. V. and Luini, A. and Polishchuk, R. S.}, citeulike-article-id = {3174917}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0076-6879(05)04005-X}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16413256}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16413256}, doi = {10.1016/S0076-6879(05)04005-X}, issn = {0076-6879}, journal = {Methods in enzymology}, keywords = {chemical\_fixation, clem, dab, gfp, hrp, live\_cell, locator\_grid, nanogold}, pages = {43--57}, posted-at = {2008-08-30 14:32:38}, priority = {2}, title = {Visualizing intracellular events in vivo by combined video fluorescence and 3-D electron microscopy.}, url = {http://dx.doi.org/10.1016/S0076-6879(05)04005-X}, volume = {404}, year = {2005} }

TY - JOUR ID - citeulike:3174917 L3 - citeulike-article-id:3174917 N2 - The combination of the capability of in vivo fluorescence video microscopy with the power of resolution of electron microscopy (EM) has been described. This approach is based on such an association of two techniques. An individual intracellular structure can be monitored in vivo, typically through the use of markers fused with green fluorescent protein (GFP), and a "snapshot" of its three-dimensional (3-D) ultrastructure and especially tomographic reconstruction can then be taken at any chosen time during its life cycle. The pitfalls and potential of this approach are discussed. JF - Methods in enzymology SN - 0076-6879 AD - Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), Italy. EP - 57 TI - Visualizing intracellular events in vivo by combined video fluorescence and 3-D electron microscopy. VL - 404 SP - 43 KW - chemical_fixation KW - clem KW - dab KW - gfp KW - hrp KW - live_cell KW - locator_grid KW - nanogold AU - Mironov, AA AU - Beznoussenko, GV AU - Luini, A AU - Polishchuk, RS PY - 2005/// UR - http://dx.doi.org/10.1016/S0076-6879(05)04005-X ER -