Light and electron microscopic localization of multiple proteins using quantum dots. Export

@article{citeulike:3179406, abstract = {Our understanding of basic cell structure and function has been greatly aided by the identification of proteins at the ultrastructural level. However, the current methods for high-resolution labeling of proteins in situ, and for directly correlating observations made by light microscopy (LM) and electron microscopy (EM) although invaluable, have a number of substantial limitations. These range from poor label penetration, difficulty to perform simultaneous multiprotein labeling, or the need to take the samples all the way to the electron microscope to evaluate labeling efficacy. Here we demonstrate an approach using quantum dots for pre-embedding immunolabeling of multiple diverse proteins for both LM and EM that overcomes many of these problems.}, address = {Department of Neurosciences, The National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, California, USA.}, author = {Deerinck, T. J. and Giepmans, B. N. and Smarr, B. L. and Martone, M. E. and Ellisman, M. H.}, citeulike-article-id = {3179406}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/17237528}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=17237528}, issn = {1064-3745}, journal = {Methods in molecular biology (Clifton, N.J.)}, keywords = {antibody, bioconjugation, clem, quantum-dots}, pages = {43--53}, posted-at = {2008-09-01 15:55:27}, priority = {2}, title = {Light and electron microscopic localization of multiple proteins using quantum dots.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17237528}, volume = {374}, year = {2007} }

TY - JOUR ID - citeulike:3179406 L3 - citeulike-article-id:3179406 N2 - Our understanding of basic cell structure and function has been greatly aided by the identification of proteins at the ultrastructural level. However, the current methods for high-resolution labeling of proteins in situ, and for directly correlating observations made by light microscopy (LM) and electron microscopy (EM) although invaluable, have a number of substantial limitations. These range from poor label penetration, difficulty to perform simultaneous multiprotein labeling, or the need to take the samples all the way to the electron microscope to evaluate labeling efficacy. Here we demonstrate an approach using quantum dots for pre-embedding immunolabeling of multiple diverse proteins for both LM and EM that overcomes many of these problems. JF - Methods in molecular biology (Clifton, N.J.) SN - 1064-3745 AD - Department of Neurosciences, The National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, California, USA. EP - 53 TI - Light and electron microscopic localization of multiple proteins using quantum dots. VL - 374 SP - 43 KW - antibody KW - bioconjugation KW - clem KW - quantum-dots AU - Deerinck, TJ AU - Giepmans, BN AU - Smarr, BL AU - Martone, ME AU - Ellisman, MH PY - 2007/// UR - http://view.ncbi.nlm.nih.gov/pubmed/17237528 ER -