Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome Export

@article{citeulike:4493866, abstract = {Visibly fluorescent proteins (FPs) from jellyfish and corals have revolutionized many areas of molecular and cell biology, but the use of FPs in intact animals, such as mice, has been handicapped by poor penetration of excitation light. We now show that a bacteriophytochrome from Deinococcus radiodurans, incorporating biliverdin as the chromophore, can be engineered into monomeric, infrared-fluorescent proteins (IFPs), with excitation and emission maxima of 684 and 708 nm, respectively; extinction coefficient >90,000 M-1 cm-1; and quantum yield of 0.07. IFPs express well in mammalian cells and mice and spontaneously incorporate biliverdin, which is ubiquitous as the initial intermediate in heme catabolism but has negligible fluorescence by itself. Because their wavelengths penetrate tissue well, IFPs are suitable for whole-body imaging. The IFPs developed here provide a scaffold for further engineering. 10.1126/science.1168683}, author = {Shu, Xiaokun and Royant, Antoine and Lin, Michael Z. and Aguilera, Todd A. and Lev-Ram, Varda and Steinbach, Paul A. and Tsien, Roger Y.}, citeulike-article-id = {4493866}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1168683}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/324/5928/804}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19423828}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19423828}, day = {8}, doi = {10.1126/science.1168683}, issn = {1095-9203}, journal = {Science}, keywords = {fluorescence, ifp, infrared, tsien}, month = {May}, number = {5928}, pages = {804--807}, posted-at = {2009-07-03 10:29:33}, priority = {2}, title = {Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome}, url = {http://dx.doi.org/10.1126/science.1168683}, volume = {324}, year = {2009} }

TY - JOUR ID - citeulike:4493866 L3 - citeulike-article-id:4493866 N2 - Visibly fluorescent proteins (FPs) from jellyfish and corals have revolutionized many areas of molecular and cell biology, but the use of FPs in intact animals, such as mice, has been handicapped by poor penetration of excitation light. We now show that a bacteriophytochrome from Deinococcus radiodurans, incorporating biliverdin as the chromophore, can be engineered into monomeric, infrared-fluorescent proteins (IFPs), with excitation and emission maxima of 684 and 708 nm, respectively; extinction coefficient >90,000 M-1 cm-1; and quantum yield of 0.07. IFPs express well in mammalian cells and mice and spontaneously incorporate biliverdin, which is ubiquitous as the initial intermediate in heme catabolism but has negligible fluorescence by itself. Because their wavelengths penetrate tissue well, IFPs are suitable for whole-body imaging. The IFPs developed here provide a scaffold for further engineering. 10.1126/science.1168683 IS - 5928 JF - Science SN - 1095-9203 EP - 807 TI - Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome VL - 324 SP - 804 KW - fluorescence KW - ifp KW - infrared KW - tsien AU - Shu, Xiaokun AU - Royant, Antoine AU - Lin, Michael AU - Aguilera, Todd AU - Lev-Ram, Varda AU - Steinbach, Paul AU - Tsien, Roger PY - 2009/05/08/ UR - http://dx.doi.org/10.1126/science.1168683 ER -