You're one in a googol: optimizing genes for protein expression Export

@article{citeulike:4643669, abstract = {10.1098/rsif.2008.0520.focus A vast number of different nucleic acid sequences can all be translated by the genetic code into the same amino acid sequence. These sequences are not all equally useful however; the exact sequence chosen can have profound effects on the expression of the encoded protein. Despite the importance of protein-coding sequences, there has been little systematic study to identify parameters that affect expression. This is probably because protein expression has largely been tackled on an ad hoc basis in many independent projects: once a sequence has been obtained that yields adequate expression for that project, there is little incentive to continue work on the problem. Synthetic biology may now provide the impetus to transform protein expression folklore into design principles, so that DNA sequences may easily be designed to express any protein in any system. In this review, we offer a brief survey of the literature, outline the major challenges in interpreting existing data and constructing robust design algorithms, and propose a way to proceed towards the goal of rational sequence engineering.}, author = {Welch, Mark and Villalobos, Alan and Gustafsson, Claes and Minshull, Jeremy}, citeulike-article-id = {4643669}, citeulike-linkout-0 = {http://dx.doi.org/10.1098/rsif.2008.0520.focus}, citeulike-linkout-1 = {http://rsif.royalsocietypublishing.org/content/rsif.2008.0520.focusv1//.abstract}, citeulike-linkout-2 = {http://rsif.royalsocietypublishing.org/content/rsif.2008.0520.focusv1//.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19324676}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19324676}, day = {6}, doi = {10.1098/rsif.2008.0520.focus}, journal = {Journal of The Royal Society Interface}, keywords = {codon\_optimization, expression, hetrologous\_expression}, month = {August}, number = {Suppl 4}, pages = {S467--S476}, posted-at = {2009-06-11 15:34:38}, priority = {2}, title = {You're one in a googol: optimizing genes for protein expression}, url = {http://dx.doi.org/10.1098/rsif.2008.0520.focus}, volume = {6}, year = {2009} }

TY - JOUR ID - citeulike:4643669 L3 - citeulike-article-id:4643669 N2 - 10.1098/rsif.2008.0520.focus A vast number of different nucleic acid sequences can all be translated by the genetic code into the same amino acid sequence. These sequences are not all equally useful however; the exact sequence chosen can have profound effects on the expression of the encoded protein. Despite the importance of protein-coding sequences, there has been little systematic study to identify parameters that affect expression. This is probably because protein expression has largely been tackled on an ad hoc basis in many independent projects: once a sequence has been obtained that yields adequate expression for that project, there is little incentive to continue work on the problem. Synthetic biology may now provide the impetus to transform protein expression folklore into design principles, so that DNA sequences may easily be designed to express any protein in any system. In this review, we offer a brief survey of the literature, outline the major challenges in interpreting existing data and constructing robust design algorithms, and propose a way to proceed towards the goal of rational sequence engineering. IS - Suppl 4 JF - Journal of The Royal Society Interface EP - S476 TI - You're one in a googol: optimizing genes for protein expression VL - 6 SP - S467 KW - codon_optimization KW - expression KW - hetrologous_expression AU - Welch, Mark AU - Villalobos, Alan AU - Gustafsson, Claes AU - Minshull, Jeremy PY - 2009/08/06/ UR - http://dx.doi.org/10.1098/rsif.2008.0520.focus ER -