Imaging Intracellular Fluorescent Proteins at Nanometer Resolution Export

@article{citeulike:838625, abstract = {We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to [\~{}]2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method--termed photoactivated localization microscopy--to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane. 10.1126/science.1127344}, address = {Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA; New Millennium Research LLC, Okemos, MI 48864, USA.}, author = {Betzig, Eric and Patterson, George H. and Sougrat, Rachid and Lindwasser, O. Wolf and Olenych, Scott and Bonifacino, Juan S. and Davidson, Michael W. and Lippincott-Schwartz, Jennifer and Hess, Harald F.}, citeulike-article-id = {838625}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1127344}, citeulike-linkout-1 = {http://www.sciencemag.org/content/313/5793/1642.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/313/5793/1642.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/313/5793/1642}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/16902090}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=16902090}, day = {15}, doi = {10.1126/science.1127344}, issn = {1095-9203}, journal = {Science}, keywords = {clem, correlative, fluorescence, palm, tirf}, month = {September}, number = {5793}, pages = {1642--1645}, posted-at = {2009-05-24 13:02:58}, priority = {2}, title = {Imaging Intracellular Fluorescent Proteins at Nanometer Resolution}, url = {http://dx.doi.org/10.1126/science.1127344}, volume = {313}, year = {2006} }

TY - JOUR ID - citeulike:838625 L3 - citeulike-article-id:838625 N2 - We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to [~]2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method--termed photoactivated localization microscopy--to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane. 10.1126/science.1127344 IS - 5793 JF - Science SN - 1095-9203 AD - Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, VA 20147, USA; New Millennium Research LLC, Okemos, MI 48864, USA. EP - 1645 TI - Imaging Intracellular Fluorescent Proteins at Nanometer Resolution VL - 313 SP - 1642 KW - clem KW - correlative KW - fluorescence KW - palm KW - tirf AU - Betzig, Eric AU - Patterson, George AU - Sougrat, Rachid AU - Lindwasser, Wolf AU - Olenych, Scott AU - Bonifacino, Juan AU - Davidson, Michael AU - Lippincott-Schwartz, Jennifer AU - Hess, Harald PY - 2006/09/15/ UR - http://dx.doi.org/10.1126/science.1127344 ER -