Toward fluorescence nanoscopy Export

@article{citeulike:911233, abstract = {For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, microscopes have been reported that provide three- to sevenfold improved axial resolution in live cells. Moreover, a family of concepts has emerged that overcomes the diffraction barrier altogether. Its first exponent, stimulated emission depletion microscopy, has so far displayed a resolution down to 28 nm. Relying on saturated optical transitions, these concepts are limited only by the attainable saturation level. As strong saturation should be feasible at low light intensities, nanoscale imaging with focused light may be closer than ever.}, address = {Max-Planck-Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 G\"{o}ttingen, Germany. hell@nanoscopy.de}, author = {Hell, Stefan W.}, citeulike-article-id = {911233}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt895}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nbt895}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/14595362}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=14595362}, day = {31}, doi = {10.1038/nbt895}, issn = {1087-0156}, journal = {Nature Biotechnology}, keywords = {4pi, fluorescence, review, ssim, sted}, month = {October}, number = {11}, pages = {1347--1355}, posted-at = {2009-05-24 13:04:05}, priority = {2}, publisher = {Nature Publishing Group}, title = {Toward fluorescence nanoscopy}, url = {http://dx.doi.org/10.1038/nbt895}, volume = {21}, year = {2003} }

TY - JOUR ID - citeulike:911233 L3 - citeulike-article-id:911233 N2 - For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, microscopes have been reported that provide three- to sevenfold improved axial resolution in live cells. Moreover, a family of concepts has emerged that overcomes the diffraction barrier altogether. Its first exponent, stimulated emission depletion microscopy, has so far displayed a resolution down to 28 nm. Relying on saturated optical transitions, these concepts are limited only by the attainable saturation level. As strong saturation should be feasible at low light intensities, nanoscale imaging with focused light may be closer than ever. IS - 11 JF - Nature Biotechnology SN - 1087-0156 AD - Max-Planck-Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany. hell@nanoscopy.de EP - 1355 TI - Toward fluorescence nanoscopy PB - Nature Publishing Group VL - 21 SP - 1347 KW - 4pi KW - fluorescence KW - review KW - ssim KW - sted AU - Hell, Stefan PY - 2003/10/31/ UR - http://dx.doi.org/10.1038/nbt895 ER -