Reassessing the role of phosphocaveolin-1 in cell adhesion and migration. Export

@article{citeulike:1686752, abstract = {Although phosphorylation on tyrosine 14 was identified early in the discovery of caveolin-1, the functional significance of this modification still remains elusive. Recent evidence points to a role of caveolin-1 tyrosine 14 phosphorylation in cell adhesion and migration. These results are based on a variety of tools, including a widely used mouse monoclonal antiphosphocaveolin-1 antibody which labels, in cultured cells, a protein localized at or near focal adhesions. We here report results from three independent laboratories, showing that this antibody recognizes phosphocaveolin-1 amongst other proteins in immunoblot analyses, and that the signal obtained with this antibody in immunostaining experiments is in part due to labeling of paxillin. Published data need to be interpreted keeping in mind that images of phosphocaveolin-1 cellular localization obtained using this antibody are not valid. We reevaluate the current knowledge about the role of caveolin-1 in cell adhesion and migration in view of this new information.}, address = {Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia.}, author = {Hill, Michelle M M. and Scherbakov, Nadja and Schiefermeier, Natalia and Baran, Joanne and Hancock, John F F. and Huber, Lukas A A. and Parton, Robert G G. and Parat, Marie-Odile O.}, citeulike-article-id = {1686752}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1600-0854.2007.00653.x}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17868074}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17868074}, day = {14}, doi = {10.1111/j.1600-0854.2007.00653.x}, issn = {1398-9219}, journal = {Traffic}, keywords = {antibodies, antibody, caveolin-1, false\_positive, focal\_adhesion, phosphorylation, staining, tyr14, valid, validity}, month = {September}, posted-at = {2007-09-23 12:30:00}, priority = {5}, title = {Reassessing the role of phosphocaveolin-1 in cell adhesion and migration.}, url = {http://dx.doi.org/10.1111/j.1600-0854.2007.00653.x}, year = {2007} }

TY - JOUR ID - citeulike:1686752 L3 - citeulike-article-id:1686752 N2 - Although phosphorylation on tyrosine 14 was identified early in the discovery of caveolin-1, the functional significance of this modification still remains elusive. Recent evidence points to a role of caveolin-1 tyrosine 14 phosphorylation in cell adhesion and migration. These results are based on a variety of tools, including a widely used mouse monoclonal antiphosphocaveolin-1 antibody which labels, in cultured cells, a protein localized at or near focal adhesions. We here report results from three independent laboratories, showing that this antibody recognizes phosphocaveolin-1 amongst other proteins in immunoblot analyses, and that the signal obtained with this antibody in immunostaining experiments is in part due to labeling of paxillin. Published data need to be interpreted keeping in mind that images of phosphocaveolin-1 cellular localization obtained using this antibody are not valid. We reevaluate the current knowledge about the role of caveolin-1 in cell adhesion and migration in view of this new information. JF - Traffic SN - 1398-9219 AD - Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia. TI - Reassessing the role of phosphocaveolin-1 in cell adhesion and migration. KW - antibodies KW - antibody KW - caveolin-1 KW - false_positive KW - focal_adhesion KW - phosphorylation KW - staining KW - tyr14 KW - valid KW - validity AU - Hill, Michelle M AU - Scherbakov, Nadja AU - Schiefermeier, Natalia AU - Baran, Joanne AU - Hancock, John F AU - Huber, Lukas A AU - Parton, Robert G AU - Parat, Marie-Odile PY - 2007/09/14/ UR - http://dx.doi.org/10.1111/j.1600-0854.2007.00653.x ER -