Arterial shear stress regulates endothelial cell-directed migration, polarity, and morphology in confluent monolayers Export

@article{citeulike:1855616, abstract = {Hemodynamic regulation of directional endothelial cell (EC) migration implies an essential role of shear stress in governing EC polarity. Shear stress induces reorientation of the microtubule organizing center toward the leading edge of migrating cells in a Cdc42-dependent manner. We have characterized the global patterns of EC migration in confluent monolayers as a function of shear stress direction and exogenous pleiotropic factors. Results demonstrate the presence of mitogenic factors significantly affects the flow-induced dynamics of movement by prolonging the onset of monolayer quiescence up to 4 days, but not shear stress-induced morphology. In conjunction with increased motility, exogenous growth factors contributed to the directed migration of ECs in the flow direction. ECs exposed to arterial flow in serum/growth factor-free media and then supplemented with growth factors rapidly increased directional migration to 85\% of cells migrating in the direction of flow and induced an increase in the distance traveled with the flow direction. This response was modulated by the directionality of flow and inhibited by the expression of dominant-negative Par6, a major downstream effector of Cdc42-induced polarity. Shear stress-induced directed migratory polarity is modulated by exogenous growth factors and dependent on Par6 activity and shear stress direction. 10.1152/ajpheart.00534.2007}, author = {Simmers, Michael B. and Pryor, Andrew W. and Blackman, Brett R.}, citeulike-article-id = {1855616}, citeulike-linkout-0 = {http://dx.doi.org/10.1152/ajpheart.00534.2007}, citeulike-linkout-1 = {http://ajpheart.physiology.org/cgi/content/abstract/293/3/H1937}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17586613}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17586613}, day = {1}, doi = {10.1152/ajpheart.00534.2007}, journal = {Am J Physiol Heart Circ Physiol}, keywords = {algorithm, elongation, endothelial, matlab, method, migration, polarization, shape, shear}, month = {September}, number = {3}, pages = {H1937--1946}, posted-at = {2007-11-02 10:01:47}, priority = {1}, title = {Arterial shear stress regulates endothelial cell-directed migration, polarity, and morphology in confluent monolayers}, url = {http://dx.doi.org/10.1152/ajpheart.00534.2007}, volume = {293}, year = {2007} }

TY - JOUR ID - citeulike:1855616 L3 - citeulike-article-id:1855616 N2 - Hemodynamic regulation of directional endothelial cell (EC) migration implies an essential role of shear stress in governing EC polarity. Shear stress induces reorientation of the microtubule organizing center toward the leading edge of migrating cells in a Cdc42-dependent manner. We have characterized the global patterns of EC migration in confluent monolayers as a function of shear stress direction and exogenous pleiotropic factors. Results demonstrate the presence of mitogenic factors significantly affects the flow-induced dynamics of movement by prolonging the onset of monolayer quiescence up to 4 days, but not shear stress-induced morphology. In conjunction with increased motility, exogenous growth factors contributed to the directed migration of ECs in the flow direction. ECs exposed to arterial flow in serum/growth factor-free media and then supplemented with growth factors rapidly increased directional migration to 85% of cells migrating in the direction of flow and induced an increase in the distance traveled with the flow direction. This response was modulated by the directionality of flow and inhibited by the expression of dominant-negative Par6, a major downstream effector of Cdc42-induced polarity. Shear stress-induced directed migratory polarity is modulated by exogenous growth factors and dependent on Par6 activity and shear stress direction. 10.1152/ajpheart.00534.2007 IS - 3 JF - Am J Physiol Heart Circ Physiol EP - 1946 TI - Arterial shear stress regulates endothelial cell-directed migration, polarity, and morphology in confluent monolayers VL - 293 SP - H1937 KW - algorithm KW - elongation KW - endothelial KW - matlab KW - method KW - migration KW - polarization KW - shape KW - shear AU - Simmers, Michael AU - Pryor, Andrew AU - Blackman, Brett PY - 2007/09/01/ UR - http://dx.doi.org/10.1152/ajpheart.00534.2007 ER -