Caveolae are an essential component of the pathway for endothelial cell signaling associated with abrupt reduction of shear stress. Export

@article{citeulike:3521425, abstract = {Abrupt cessation of flow representing the acute loss of shear stress (simulated ischemia) to flow-adapted pulmonary microvascular endothelial cells (PMVEC) leads to reactive oxygen species (ROS) generation that signals for EC proliferation. We evaluated the role of caveolin-1 on this cellular response with mouse PMVEC that were preconditioned for 72 h to laminar flow at 5 dyn/cm(2) followed by stop of flow ("ischemia"). Preconditioning resulted in a 2.7-fold increase in cellular expression of K(ATP) (K(IR) 6.2) channels but no change in expression level of caveolin-1, gp91(phox), or MAP kinases. The initial response to ischemia in wild type cells was cell membrane depolarization that was abolished by gene targeting of K(IR) 6.2. The subsequent response was increased ROS production associated with activation of NADPH oxidase (NOX2) and then phosphorylation of MAP kinases (Erk, JNK). After 24 h of ischemia in wild type cells, the cell proliferation index increased 2.5 fold and the \% of cells in S+G(2)/M phases increased 6-fold. This signaling cascade (cell membrane depolarization, ROS production, MAP kinase activation and cell proliferation) was abrogated in caveolin-1 null PMVEC or by treatment of wild type cells with filipin. These studies indicate that caveolin-1 functions as a shear sensor in flow-adapted EC resulting in ROS-mediated cell signaling and endothelial cell proliferation following the abrupt reduction in flow.}, author = {Milovanova, T. and Chatterjee, S. and Hawkins, B. J. and Hong, N. and Sorokina, E. M. and Debolt, K. and Moore, J. S. and Madesh, M. and Fisher, A. B.}, citeulike-article-id = {3521425}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.bbamcr.2008.05.010}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18573285}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18573285}, doi = {10.1016/j.bbamcr.2008.05.010}, issn = {0006-3002}, journal = {Biochimica et biophysica acta}, keywords = {caveolae, disruption, erk12, flow, ischemia, proliferation, shear}, month = {October}, number = {10}, pages = {1866--1875}, posted-at = {2008-11-16 15:56:18}, priority = {2}, title = {Caveolae are an essential component of the pathway for endothelial cell signaling associated with abrupt reduction of shear stress.}, url = {http://dx.doi.org/10.1016/j.bbamcr.2008.05.010}, volume = {1783}, year = {2008} }

TY - JOUR ID - citeulike:3521425 L3 - citeulike-article-id:3521425 N2 - Abrupt cessation of flow representing the acute loss of shear stress (simulated ischemia) to flow-adapted pulmonary microvascular endothelial cells (PMVEC) leads to reactive oxygen species (ROS) generation that signals for EC proliferation. We evaluated the role of caveolin-1 on this cellular response with mouse PMVEC that were preconditioned for 72 h to laminar flow at 5 dyn/cm(2) followed by stop of flow ("ischemia"). Preconditioning resulted in a 2.7-fold increase in cellular expression of K(ATP) (K(IR) 6.2) channels but no change in expression level of caveolin-1, gp91(phox), or MAP kinases. The initial response to ischemia in wild type cells was cell membrane depolarization that was abolished by gene targeting of K(IR) 6.2. The subsequent response was increased ROS production associated with activation of NADPH oxidase (NOX2) and then phosphorylation of MAP kinases (Erk, JNK). After 24 h of ischemia in wild type cells, the cell proliferation index increased 2.5 fold and the % of cells in S+G(2)/M phases increased 6-fold. This signaling cascade (cell membrane depolarization, ROS production, MAP kinase activation and cell proliferation) was abrogated in caveolin-1 null PMVEC or by treatment of wild type cells with filipin. These studies indicate that caveolin-1 functions as a shear sensor in flow-adapted EC resulting in ROS-mediated cell signaling and endothelial cell proliferation following the abrupt reduction in flow. IS - 10 JF - Biochimica et biophysica acta SN - 0006-3002 EP - 1875 TI - Caveolae are an essential component of the pathway for endothelial cell signaling associated with abrupt reduction of shear stress. VL - 1783 SP - 1866 KW - caveolae KW - disruption KW - erk12 KW - flow KW - ischemia KW - proliferation KW - shear AU - Milovanova, T AU - Chatterjee, S AU - Hawkins, BJ AU - Hong, N AU - Sorokina, EM AU - Debolt, K AU - Moore, JS AU - Madesh, M AU - Fisher, AB PY - 2008/10// UR - http://dx.doi.org/10.1016/j.bbamcr.2008.05.010 ER -