Global regulation of erythroid gene expression by transcription factor GATA-1 Export

@article{citeulike:449218, abstract = {Transcription factor GATA-1 is required for erythropoiesis, yet its full actions are unknown. We performed transcriptome analysis of G1E-ER4 cells, a GATA-1-null erythroblast line that undergoes synchronous erythroid maturation when GATA-1 activity is restored. We interrogated more than 9000 transcripts at 6 time points representing the transition from late burst forming unit-erythroid (BFU-E) to basophilic erythroblast stages. Our findings illuminate several new aspects of GATA-1 function. First, the large number of genes responding quickly to restoration of GATA-1 extends the repertoire of its potential targets. Second, many transcripts were rapidly down-regulated, highlighting the importance of GATA-1 in gene repression. Third, up-regulation of some known GATA-1 targets was delayed, suggesting that auxiliary factors are required. For example, induction of the direct GATA-1 target gene {beta} major globin was late and, surprisingly, required new protein synthesis. In contrast, the gene encoding Fog1, which cooperates with GATA-1 in {beta} globin transcription, was rapidly induced independently of protein synthesis. Guided by bioinformatic analysis, we demonstrated that selected regions of the Fog1 gene exhibit enhancer activity and in vivo occupancy by GATA-1. These findings define a regulatory loop for {beta} globin expression and, more generally, demonstrate how transcriptome analysis can be used to generate testable hypotheses regarding transcriptional networks.}, author = {Welch, John J. and Watts, Jason A. and Vakoc, Christopher R. and Yao, Yu and Wang, Hao and Hardison, Ross C. and Blobel, Gerd A. and Chodosh, Lewis A. and Weiss, Mitchell J.}, citeulike-article-id = {449218}, citeulike-linkout-0 = {http://www.bloodjournal.org/cgi/content/abstract/104/10/3136}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15297311}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15297311}, day = {15}, journal = {Blood}, keywords = {erythropoiesis, microarray, transcription}, month = {November}, number = {10}, pages = {3136--3147}, posted-at = {2005-12-24 03:57:57}, priority = {2}, title = {Global regulation of erythroid gene expression by transcription factor GATA-1}, url = {http://www.bloodjournal.org/cgi/content/abstract/104/10/3136}, volume = {104}, year = {2004} }

TY - JOUR ID - citeulike:449218 L3 - citeulike-article-id:449218 N2 - Transcription factor GATA-1 is required for erythropoiesis, yet its full actions are unknown. We performed transcriptome analysis of G1E-ER4 cells, a GATA-1-null erythroblast line that undergoes synchronous erythroid maturation when GATA-1 activity is restored. We interrogated more than 9000 transcripts at 6 time points representing the transition from late burst forming unit-erythroid (BFU-E) to basophilic erythroblast stages. Our findings illuminate several new aspects of GATA-1 function. First, the large number of genes responding quickly to restoration of GATA-1 extends the repertoire of its potential targets. Second, many transcripts were rapidly down-regulated, highlighting the importance of GATA-1 in gene repression. Third, up-regulation of some known GATA-1 targets was delayed, suggesting that auxiliary factors are required. For example, induction of the direct GATA-1 target gene {beta} major globin was late and, surprisingly, required new protein synthesis. In contrast, the gene encoding Fog1, which cooperates with GATA-1 in {beta} globin transcription, was rapidly induced independently of protein synthesis. Guided by bioinformatic analysis, we demonstrated that selected regions of the Fog1 gene exhibit enhancer activity and in vivo occupancy by GATA-1. These findings define a regulatory loop for {beta} globin expression and, more generally, demonstrate how transcriptome analysis can be used to generate testable hypotheses regarding transcriptional networks. IS - 10 JF - Blood EP - 3147 TI - Global regulation of erythroid gene expression by transcription factor GATA-1 VL - 104 SP - 3136 KW - erythropoiesis KW - microarray KW - transcription AU - Welch, John AU - Watts, Jason AU - Vakoc, Christopher AU - Yao, Yu AU - Wang, Hao AU - Hardison, Ross AU - Blobel, Gerd AU - Chodosh, Lewis AU - Weiss, Mitchell PY - 2004/11/15/ UR - http://www.bloodjournal.org/cgi/content/abstract/104/10/3136 ER -