Rapid activation of plasticity-associated gene transcription in hippocampal neurons provides a mechanism for encoding of one-trial experience. Export

@article{citeulike:4026244, abstract = {The hippocampus is hypothesized to support rapid encoding of ongoing experience. A critical prerequisite for such function is the ability to readily recruit enduring synaptic plasticity in hippocampal neurons. Hippocampal long-term potentiation (LTP) and memory consolidation require expression of the immediate-early gene (IEG) Arc. To determine whether Arc transcription could be driven by limited and controlled behavioral experience, we used a rectangular track paradigm. In past electrophysiological studies, pyramidal neurons recorded from rats running in one direction on similar tracks typically exhibited a single firing field. Using fluorescence in situ hybridization, we show that the behavioral activity associated with a single lap around the track was sufficient to trigger Arc transcription in complete CA3 neuronal ensembles, as predicted given the role of CA3 in one-trial learning. In contrast, Arc transcription in CA1 ensembles was recruited incrementally, with maximal activation achieved after four laps a day for 4 consecutive days. To test whether Arc transcription is linked to learning and plasticity, or merely elicited by location-specific firing, we inactivated the medial septum, a treatment that compromises hippocampus-dependent learning and LTP but spares location-specific firing in CA1 neurons. Septal inactivation abolished track training-induced Arc transcription in CA1 and CA3 neurons, showing that Arc transcription requires plasticity-inducing stimuli. Accordingly, LTP induction activated Arc transcription in CA1 neurons in vivo. These findings demonstrate for the first time that a single brief experience, equivalent to a single crossing of a firing field, can trigger IEG expression required for long-term plasticity in the hippocampus.}, author = {Miyashita, T. and Kubik, S. and Haghighi, N. and Steward, O. and Guzowski, J. F.}, citeulike-article-id = {4026244}, citeulike-linkout-0 = {http://dx.doi.org/10.1523/JNEUROSCI.4588-08.2009}, citeulike-linkout-1 = {http://www.jneurosci.org/cgi/content/abstract/29/4/898}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19176799}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19176799}, day = {28}, doi = {10.1523/JNEUROSCI.4588-08.2009}, issn = {1529-2401}, journal = {The Journal of neuroscience : the official journal of the Society for Neuroscience}, keywords = {100209, arc, genetics, hippocampus, learning\_and\_memory}, month = {January}, number = {4}, pages = {898--906}, posted-at = {2009-02-09 19:07:10}, priority = {2}, title = {Rapid activation of plasticity-associated gene transcription in hippocampal neurons provides a mechanism for encoding of one-trial experience.}, url = {http://dx.doi.org/10.1523/JNEUROSCI.4588-08.2009}, volume = {29}, year = {2009} }

TY - JOUR ID - citeulike:4026244 L3 - citeulike-article-id:4026244 N2 - The hippocampus is hypothesized to support rapid encoding of ongoing experience. A critical prerequisite for such function is the ability to readily recruit enduring synaptic plasticity in hippocampal neurons. Hippocampal long-term potentiation (LTP) and memory consolidation require expression of the immediate-early gene (IEG) Arc. To determine whether Arc transcription could be driven by limited and controlled behavioral experience, we used a rectangular track paradigm. In past electrophysiological studies, pyramidal neurons recorded from rats running in one direction on similar tracks typically exhibited a single firing field. Using fluorescence in situ hybridization, we show that the behavioral activity associated with a single lap around the track was sufficient to trigger Arc transcription in complete CA3 neuronal ensembles, as predicted given the role of CA3 in one-trial learning. In contrast, Arc transcription in CA1 ensembles was recruited incrementally, with maximal activation achieved after four laps a day for 4 consecutive days. To test whether Arc transcription is linked to learning and plasticity, or merely elicited by location-specific firing, we inactivated the medial septum, a treatment that compromises hippocampus-dependent learning and LTP but spares location-specific firing in CA1 neurons. Septal inactivation abolished track training-induced Arc transcription in CA1 and CA3 neurons, showing that Arc transcription requires plasticity-inducing stimuli. Accordingly, LTP induction activated Arc transcription in CA1 neurons in vivo. These findings demonstrate for the first time that a single brief experience, equivalent to a single crossing of a firing field, can trigger IEG expression required for long-term plasticity in the hippocampus. IS - 4 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience SN - 1529-2401 EP - 906 TI - Rapid activation of plasticity-associated gene transcription in hippocampal neurons provides a mechanism for encoding of one-trial experience. VL - 29 SP - 898 KW - 100209 KW - arc KW - genetics KW - hippocampus KW - learning_and_memory AU - Miyashita, T AU - Kubik, S AU - Haghighi, N AU - Steward, O AU - Guzowski, JF PY - 2009/01/28/ UR - http://dx.doi.org/10.1523/JNEUROSCI.4588-08.2009 ER -