Functional Versatility and Molecular Diversity of the Metabolic Map of Escherichia coli Export

@article{citeulike:387967, abstract = {10.1101/gr.187501 We have analyzed the known metabolic enzymes of in relation to their biochemical reaction properties and their involvement in biochemical pathways. All enzymes involved in small-molecule metabolism and their corresponding protein sequences have been extracted from the EcoCyc database. These 548 metabolic enzymes are clustered into 405 protein families according to sequence similarity. In this study, we examine the functional versatility within enzyme families in terms of their reaction capabilities and pathway participation. In addition, we examine the molecular diversity of reactions and pathways according to their presence across enzyme families. These complex, many-to-many relationships between protein sequence and biochemical function reveal a significant degree of correlation between enzyme families and reactions. Pathways, however, appear to require more than one enzyme type to perform their complex biochemical transformations. Finally, the distribution of enzyme family members across different pathways provides support for the \^{a}€œrecruitment\^{a}€ hypothesis of biochemical pathway evolution.}, address = {Computational Genomics Group, Research Programme, The European Bioinformatics Institute, European Molecular Biology Laboratory, Cambridge Outstation, Cambridge CB10 1SD, UK.}, author = {Tsoka, Sophia and Ouzounis, Christos A.}, citeulike-article-id = {387967}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gr.187501}, citeulike-linkout-1 = {http://genome.cshlp.org/content/11/9/1503.abstract}, citeulike-linkout-2 = {http://genome.cshlp.org/content/11/9/1503.full.pdf}, citeulike-linkout-3 = {http://www.genome.org/cgi/content/abstract/11/9/1503}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/11544193}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=11544193}, day = {1}, doi = {10.1101/gr.187501}, issn = {1088-9051}, journal = {Genome Research}, month = {September}, number = {9}, pages = {1503--1510}, posted-at = {2009-10-12 11:35:50}, priority = {0}, title = {Functional Versatility and Molecular Diversity of the Metabolic Map of Escherichia coli}, url = {http://dx.doi.org/10.1101/gr.187501}, volume = {11}, year = {2001} }

TY - JOUR ID - citeulike:387967 L3 - citeulike-article-id:387967 N2 - 10.1101/gr.187501 We have analyzed the known metabolic enzymes of in relation to their biochemical reaction properties and their involvement in biochemical pathways. All enzymes involved in small-molecule metabolism and their corresponding protein sequences have been extracted from the EcoCyc database. These 548 metabolic enzymes are clustered into 405 protein families according to sequence similarity. In this study, we examine the functional versatility within enzyme families in terms of their reaction capabilities and pathway participation. In addition, we examine the molecular diversity of reactions and pathways according to their presence across enzyme families. These complex, many-to-many relationships between protein sequence and biochemical function reveal a significant degree of correlation between enzyme families and reactions. Pathways, however, appear to require more than one enzyme type to perform their complex biochemical transformations. Finally, the distribution of enzyme family members across different pathways provides support for the “recruitment” hypothesis of biochemical pathway evolution. IS - 9 JF - Genome Research SN - 1088-9051 AD - Computational Genomics Group, Research Programme, The European Bioinformatics Institute, European Molecular Biology Laboratory, Cambridge Outstation, Cambridge CB10 1SD, UK. EP - 1510 TI - Functional Versatility and Molecular Diversity of the Metabolic Map of Escherichia coli VL - 11 SP - 1503 AU - Tsoka, Sophia AU - Ouzounis, Christos PY - 2001/09/01/ UR - http://dx.doi.org/10.1101/gr.187501 ER -