Haemoglobin immobilized on nafion modified multi-walled carbon nanotubes for O2, H2O2 and CCl3COOH sensors Export

@article{citeulike:4179596, abstract = {A conductive biocomposite film (MWCNTs-NF-Hb) containing multi-walled carbon nanotubes (MWCNTs) incorporated with entrapped haemoglobin (Hb) in nafion (NF) has been synthesized on glassy carbon electrode (GCE), gold (Au), indium tin oxide (ITO) and screen printed carbon electrode (SPCE) separately by potentiostatic methods. The presence of both MWCNTs and NF in the biocomposite film enhances the surface coverage concentration ( Γ ), and increases the electron transfer rate constant ( K s ) to 132\%. The biocomposite film exhibits a promising enhanced electrocatalytic activity towards the reduction of O 2 , H 2 O 2 and CCl 3 COOH. The cyclic voltammetry has been used for the measurement of electrocatalysis results of analytes by means of biocomposite film-modified GCEs. The MWCNTs-NF-Hb-modified GCEs' sensitivity values are higher than the values obtained for other film modified GCEs. The surface morphology of the biocomposite films which have been deposited on ITO has been studied using scanning electron microscopy and atomic force microscopy. The studies have revealed that there was an incorporation of NF and immobilization of Hb on MWCNTs. Finally, the flow injection analysis has been used for the amperometric studies of analytes at MWCNTs-Hb and MWCNTs-NF-Hb film modified SPCEs. The amperometric study results have shown higher slope values for MWCNTs-NF-Hb biocomposite film.}, author = {Shie, Jan-Wei and Umasankar, Yogeswaran and Chen, Shen-Ming}, citeulike-article-id = {4179596}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.talanta.2008.12.063}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0039914009000472}, day = {15}, doi = {10.1016/j.talanta.2008.12.063}, issn = {00399140}, journal = {Talanta}, keywords = {acid, carbon, electrocatalysis, electrodes, hydrogen, modified, multiwall, nanotubes, oxygen, peroxide, trichloroacetic}, month = {May}, number = {3}, pages = {896--902}, posted-at = {2009-03-15 18:22:20}, priority = {2}, title = {Haemoglobin immobilized on nafion modified multi-walled carbon nanotubes for O2, H2O2 and CCl3COOH sensors}, url = {http://dx.doi.org/10.1016/j.talanta.2008.12.063}, volume = {78}, year = {2009} }

TY - JOUR ID - citeulike:4179596 L3 - citeulike-article-id:4179596 N2 - A conductive biocomposite film (MWCNTs-NF-Hb) containing multi-walled carbon nanotubes (MWCNTs) incorporated with entrapped haemoglobin (Hb) in nafion (NF) has been synthesized on glassy carbon electrode (GCE), gold (Au), indium tin oxide (ITO) and screen printed carbon electrode (SPCE) separately by potentiostatic methods. The presence of both MWCNTs and NF in the biocomposite film enhances the surface coverage concentration ( Γ ), and increases the electron transfer rate constant ( K s ) to 132%. The biocomposite film exhibits a promising enhanced electrocatalytic activity towards the reduction of O 2 , H 2 O 2 and CCl 3 COOH. The cyclic voltammetry has been used for the measurement of electrocatalysis results of analytes by means of biocomposite film-modified GCEs. The MWCNTs-NF-Hb-modified GCEs’ sensitivity values are higher than the values obtained for other film modified GCEs. The surface morphology of the biocomposite films which have been deposited on ITO has been studied using scanning electron microscopy and atomic force microscopy. The studies have revealed that there was an incorporation of NF and immobilization of Hb on MWCNTs. Finally, the flow injection analysis has been used for the amperometric studies of analytes at MWCNTs-Hb and MWCNTs-NF-Hb film modified SPCEs. The amperometric study results have shown higher slope values for MWCNTs-NF-Hb biocomposite film. IS - 3 JF - Talanta SN - 00399140 EP - 902 TI - Haemoglobin immobilized on nafion modified multi-walled carbon nanotubes for O2, H2O2 and CCl3COOH sensors VL - 78 SP - 896 KW - acid KW - carbon KW - electrocatalysis KW - electrodes KW - hydrogen KW - modified KW - multiwall KW - nanotubes KW - oxygen KW - peroxide KW - trichloroacetic AU - Shie, Jan-Wei AU - Umasankar, Yogeswaran AU - Chen, Shen-Ming PY - 2009/05/15/ UR - http://dx.doi.org/10.1016/j.talanta.2008.12.063 ER -