Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks Export

@article{citeulike:1048021, abstract = {Abstract\ \ In spite of strong evidence that the nucleus is a highly organized organelle, a consensus on basic principles of the global nuclear architecture has not so far been achieved. The chromosome territory–interchromatin compartment (CT-IC) model postulates an IC which expands between chromatin domains both in the interior and the periphery of CT. Other models, however, dispute the existence of the IC and claim that numerous chromatin loops expand between and within CTs. The present study was undertaken to resolve these conflicting views. (1) We demonstrate that most chromatin exists in the form of higher-order chromatin domains with a compaction level at least 10 times above the level of extended 30\ nm chromatin fibers. A similar compaction level was obtained in a detailed analysis of a particularly gene-dense chromosome region on HSA 11, which often expanded from its CT as a finger-like chromatin protrusion. (2) We further applied an approach which allows the experimental manipulation of both chromatin condensation and the width of IC channels in a fully reversible manner. These experiments, together with electron microscopic observations, demonstrate the existence of the IC as a dynamic, structurally distinct nuclear compartment, which is functionally linked with the chromatin compartment.}, author = {Albiez, Heiner and Cremer, Marion and Tiberi, Cinzia and Vecchio, Lorella and Schermelleh, Lothar and Dittrich, Sandra and K\"{u}pper, Katrin and Joffe, Boris and Thormeyer, Tobias and von Hase, Johann and Yang, Siwei and Rohr, Karl and Leonhardt, Heinrich and Solovei, Irina and Cremer, Christoph and Fakan, Stanislav and Cremer, Thomas}, citeulike-article-id = {1048021}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s10577-006-1086-x}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/klu/chro/2006/00000014/00000007/00001086}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17115328}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17115328}, citeulike-linkout-4 = {http://www.springerlink.com/content/c56vtm229547348w}, day = {1}, doi = {10.1007/s10577-006-1086-x}, issn = {0967-3849}, journal = {Chromosome Research}, keywords = {chromatin, interchromatin\_domain\_compartment, interchromatine\_domain\_compartment, nuclear\_matrix, nucleus, somatic}, month = {November}, number = {7}, pages = {707--733}, posted-at = {2007-05-02 16:11:51}, priority = {4}, publisher = {Springer}, title = {Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks}, url = {http://dx.doi.org/10.1007/s10577-006-1086-x}, volume = {14}, year = {2006} }

TY - JOUR ID - citeulike:1048021 L3 - citeulike-article-id:1048021 N2 - Abstract  In spite of strong evidence that the nucleus is a highly organized organelle, a consensus on basic principles of the global nuclear architecture has not so far been achieved. The chromosome territory–interchromatin compartment (CT-IC) model postulates an IC which expands between chromatin domains both in the interior and the periphery of CT. Other models, however, dispute the existence of the IC and claim that numerous chromatin loops expand between and within CTs. The present study was undertaken to resolve these conflicting views. (1) We demonstrate that most chromatin exists in the form of higher-order chromatin domains with a compaction level at least 10 times above the level of extended 30 nm chromatin fibers. A similar compaction level was obtained in a detailed analysis of a particularly gene-dense chromosome region on HSA 11, which often expanded from its CT as a finger-like chromatin protrusion. (2) We further applied an approach which allows the experimental manipulation of both chromatin condensation and the width of IC channels in a fully reversible manner. These experiments, together with electron microscopic observations, demonstrate the existence of the IC as a dynamic, structurally distinct nuclear compartment, which is functionally linked with the chromatin compartment. IS - 7 JF - Chromosome Research SN - 0967-3849 EP - 733 TI - Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks PB - Springer VL - 14 SP - 707 KW - chromatin KW - interchromatin_domain_compartment KW - interchromatine_domain_compartment KW - nuclear_matrix KW - nucleus KW - somatic AU - Albiez, Heiner AU - Cremer, Marion AU - Tiberi, Cinzia AU - Vecchio, Lorella AU - Schermelleh, Lothar AU - Dittrich, Sandra AU - Küpper, Katrin AU - Joffe, Boris AU - Thormeyer, Tobias AU - von Hase, Johann AU - Yang, Siwei AU - Rohr, Karl AU - Leonhardt, Heinrich AU - Solovei, Irina AU - Cremer, Christoph AU - Fakan, Stanislav AU - Cremer, Thomas PY - 2006/11/01/ UR - http://dx.doi.org/10.1007/s10577-006-1086-x ER -