Presence of two deoxyribonucleic acid polymerases in bull spermatozoa. Export

@article{citeulike:1140527, abstract = {A DNA polymerase-endogenous template complex was isolated from nuclear heads of bull spermatozoa. The buoyant density of the complex was 1.15 g/cm 3. The sedimentation coefficient of the nuclear DNA polymerase isolated from the complex was higher at low ionic strength, but approached 3.4S when centrifuged in a medium containing 2M-KCl. Activated exogenous DNA increased polymerase activity. Only very low activities were detected with synthetic templates such as poly(A).(dT)12-18 and poly(dT).poly(A). The nuclear reaction was stimulated by 150mM-KCl and was slightly inhibited by N-ethylmaleimide; it was resistant to actinomycin D, netropsin and ethidium bromide. Another DNA polymerase, highly sensitive to ethidium bromide, was extracted from the mitochondira-rich middle-piece fraction. Its sedimentation coefficient was close to 9S, but fell to approx. 4S in high-ionic-strength medium.}, author = {Philippe, M. and Chevaillier, P.}, citeulike-article-id = {1140527}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/743212}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=743212}, day = {1}, issn = {0264-6021}, journal = {Biochem J}, keywords = {bovine, bull, dna\_polymerase, spermatozoa}, month = {November}, number = {2}, pages = {595--600}, posted-at = {2007-03-05 05:41:25}, priority = {1}, title = {Presence of two deoxyribonucleic acid polymerases in bull spermatozoa.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/743212}, volume = {175}, year = {1978} }

TY - JOUR ID - citeulike:1140527 L3 - citeulike-article-id:1140527 N2 - A DNA polymerase-endogenous template complex was isolated from nuclear heads of bull spermatozoa. The buoyant density of the complex was 1.15 g/cm 3. The sedimentation coefficient of the nuclear DNA polymerase isolated from the complex was higher at low ionic strength, but approached 3.4S when centrifuged in a medium containing 2M-KCl. Activated exogenous DNA increased polymerase activity. Only very low activities were detected with synthetic templates such as poly(A).(dT)12-18 and poly(dT).poly(A). The nuclear reaction was stimulated by 150mM-KCl and was slightly inhibited by N-ethylmaleimide; it was resistant to actinomycin D, netropsin and ethidium bromide. Another DNA polymerase, highly sensitive to ethidium bromide, was extracted from the mitochondira-rich middle-piece fraction. Its sedimentation coefficient was close to 9S, but fell to approx. 4S in high-ionic-strength medium. IS - 2 JF - Biochem J SN - 0264-6021 EP - 600 TI - Presence of two deoxyribonucleic acid polymerases in bull spermatozoa. VL - 175 SP - 595 KW - bovine KW - bull KW - dna_polymerase KW - spermatozoa AU - Philippe, M AU - Chevaillier, P PY - 1978/11/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/743212 ER -